• Nesti Fronika Sianipar Department of Food Technology, Faculty of Engineering Bina Nusantara University, Tangerang 15325, Indonesia
  • Ragapadmi Purnamaningsih Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB-Biogen), Bogor 16111, Indonesia
  • Chelen Chelen Student Alumna of Biology Department, Faculty of Science and Technology, Universitas Pelita Harapan, Tangerang15811, Indonesia




Typhonium flagelliforme Lodd., in vitro mutant, 1D PAGE, 2D PAGE, gamma irradiation


Rodent tuber is an Indonesian anticancer herbal plant whose genetic variation is low. Genetic variation is crucial to be increased to increase the content of anticancer bioactive compounds. In vitro calli of rodent tuber had been mutated by gamma irradiation. Mutant plants had their genome modified and therefore also affected protein expression. The purpose of this research is to identify protein expression differences between mutant and control rodent tuber plants by 1D and 2D PAGE. 1D PAGE had shown differences in the number of protein bands between control and mutant. M6/2 did not show any expression of 6,9 kD protein. 2D PAGE had identified 24 proteins which were expressed by control and M6/2 plants. Among them, 13 spots were up-regulated and 9 spots were down-regulated in M6/2 plants. In conclusion, 1D and 2D PAGE analyses had successfully proven the changes in protein expression between control and mutant rodent tuber plants.  


Essai. 1985. Medicinal Herbs Index In Indonesia. Jakarta: PT Essai Indonesia.

Syahid, S. F. 2008. Keragaman Morfologi, Pertumbuhan, Produksi, Mutu Dan Fitokimia Keladi Tikus (Typonium Flagelliforme Lodd.) Blume Asal Variasi Somaklonal. Jurnal Littri. 14(3): 113-118.

Nobakht, G. M., Kadir, M. A. and Stanslas, J. 2010. Analysis Of Preliminary Phytochemical Screening Of Typhonium Flagelliforme. African Journal of Biotechnology. 9: 1655-1657.

Farida, Y., Wahyuni, P. S, Wahono, S. and Hanafi, M. 2012. Flavonoid Glycoside From The Ethyl Acetate Extract Of Keladi Tikus Typhonium Flagelliforme Lodd. Blume Leaves. Asian Journal Of Natural & Applied Science. 1(4).

Lai, C. S., Mas, R. H, Nair, N. K, Majid, M.I, Mansor, S. M, and Navaratnam, V. 2008. Typhonium flagelliforme Inhibits Cancer Cell Growth In Vitro And Induces Apoptosis: An Evaluation By The Bioactivity Guided Approach. Journal of Ethnopharmacology. 118(1): 14-20.

Mohan, S., Abdul, A. B., Abdelwahab, S. I., Al-Zubairi, A. S., Aspollah, Sukari, M., Abdullah, R., Taha, M. M., Beng, N. K., and Isa, N. M. 2010. Typhonium Flagelliforme Inhibits The Proliferation Of Murine Leukemia WEHI-3 Cells In Vitro And Induces Apoptosis In Vivo. Leukimia Research. 34 (11): 1483-1492.

Essai. 1986. Medicinal Herbs Index In Indonesia. Jakarta: PT Essai Indonesia.

Sianipar, N. F., Rustikawati, Maarisit, W., Wantho, A., and Sidabutar, D. N..R. 2011. Embryonic Calli Induction, Proliferation, And Regeneration Of Rodent Tuber Plant (Typhonium Flagelliforme Lodd.) By Single Node Culture. International Conference on Biological Science, Faculty of Biology, Universitas Gadjah Mada. 80-88.

Sianipar, N. F., Wantho, A, Rustikawati, and Maarisit, W. 2013. Effects of Gamma Irradiation and Mutant Morphology of In Vitro Culture of Rodent Tuber (Typhonium flagelliforme Lodd.). Hayati Journal Of Biosciences. 20: 51-56.

Natawijaya, A., Afiyata, A, and Ritonga, A.W. 2009. Pengaruh Iradiasi Sinar Gamma terhadap Keragaan Planlet Tanaman Gloxinia. Skripsi. Program Studi Pemuliaan dan Bioteknologi Tanaman Departemen Agronomi dan Hortikultura. Sekolah Pasca Sarjana. Bogor: Institut Pertanian Bogor.

Wegadara, M. 2008. Pengaruh Iradiasi Sinar Gamma pada Buah terhadap Keragaan Tanaman Anthurium (Anthurium andreanum). Skripsi. Program Studi Pemuliaan dan Tanaman dan Teknologi Benih. Fakultas Pertanian. Bogor: Institut Pertanian Bogor.

Karp, G. 2008. Cell and Molecular Biology – Concept and Experiment 5th. USA: John Wiley & Sons, Inc.

Kovacs, E. and Keresztes, A. 2002. Effect Of Gamma And UV-B/C Radiation On Plant Cells. Micron. 33: 199-210.

Smolka, M., Zhou, H, and Aebersold, R. 2002. Quantitative Protein Profiling Using Two-dimensional Gel Electrophoresis, Isotope-coded Affinity Tag Labeling, and Mass Spectrometry. Molecular & Cellular Proteomics. 1: 19–29.

Listyari, B. P. 2006. Analisis Diosmin Dan Protein Tanaman Seledri (Apium graveolens L.) dari daerah Cipanas dan Ciwidey. Skripsi. Program Studi Biokimia Fakultas Matematika dan Ilmu Pengetahuan Alam. Bogor: Institut Pertanian Bogor.

Fatmawati, D. A. 2008. Pola Protein dan Kandungan Kurkuminoid Rimpang Temulawak. Skripsi. Program Studi Biokimia. Fakultas Matematika dan Ilmu Pengetahuan Alam. Bogor: Institut Pertanian Bogor.

Saini, D., and Sarin, R. 2012. SDS-PAGE Analysis of Leaf Galls of Alstonia scholaris (L.) R. Journal Plant Pathol Microb. 3(2).

Garfin, D. E. 2003. Essential Cell Biology: Cell Structure. Oxford: Oxford University Press.

Issaq, H, and Veesentra T. 2008. Two-Dimensional Ployacrylamide Gel Electrophoresis (2D PAGE): Advance And Perspective. Mini Review. Biotechniques. 44: 697-700.

Rout, J. R., Kanungo, S., Das, R., and Sahoo, S. L. 2010. In Vivo Protein Profiling And Catalase Activity Of Plumbago Zeylanica L. Nature And Science. 8: 87-90.

Lamely, U. K. 1970. Cleavage Of Structural Protein During The Assembly Of The Head Bacteriophage T4. Nature. 227: 680-685.

Chan, L. K., Koh, W. Y, and Tengku-Muhammad, S. T. 2005. Comparison Of Cytotoxic Activities Between In Vitro And Field Grown Plants Of Typhonium Flagelliforme (Lodd.) Blume. Journal of Plant Biology. 48: 25-31.

Nobakht, G. M., Kadir, M. A., and Stanslas, J. 2009. In Vitro Mass Propagation Of Typhonium Flagelli- Forme As Affected By Plant Growth Regulator. African Journal of Biotechnology. 8: 6840-6843.

Larkin, P. J, and Scowcroft, W.R. 1981. Somaclonal Variation, A Novel Source Of Variability From Cell For Plant Improvement. Theoretical and Applied Genetics. 60: 197-214.

Van Harten, A. M. 1998. Mutation Breeding: Theory and Practical Applications. Cambridge: Cambridge university Press.

Sianipar, N. F., Wantho, A., Rustikawati, and Maarisit, W. 2015. Detection of Gamma-Irradiated Mu- tant of Rodent Tuber (Typhonium flagelliforme Lodd.) In Vitro Culture by RAPD Molecular Marker. Elsevier Procedia Chemistry. 14: 285-294.

Ng, P. C, and Henikoff, S. 2006. Predicting the Effects of Amino Acid Substitutions on Protein Function. Annual Review of Genomics and Human Genetics. 7: 61-80.

Sun, L., Lei, X., Ma, R., Jiang, R., Zhao, D., and Wang, Y. 2011. Two Dimensional Gel Electropho- resis Analysis of Different Parts of Panax quinquefolius L. Root. African journal of Biotechnology. 10(74): 17023-17029.

Chatterjee, Gupta, S., Bhar, A. and Das, S. 2012. Optimization of an Efficient Protein Extrac- tion Protocol Compatible with Two Dimensional Electrophoresis and Mass Spectrometry from Recalcitrant Phenolic Rich Roots of Chickpea (Cicer arietinum L.). International Journal Of Proteomics. doi: 10.1155/2012/536963

Faurobert, M., Pelpoir, E, and Chaib, J. 2007. Phenol Extraction Of Proteins For Proteomic Studies. New Jersey: Plant Proteomic Human press.

Marsoni, M., Vannini, C, Campa, M., Cucchi, U., Espen, L., and Bracale, M. 2005. Protein Extraction from Grape Tissue by Two Dimensional Electrophoresis. Vitis. 44(4): 181-186.

Kiong, A. L. P., Lai, A. G., Hussein, S., and Harun, A. R. 2008. Physiological Responses of Orthosi- phon stamineus Plantles to Gamma Irradiation. American-Eurasian Journal of Sustainable Agriculture. C(C): 1-15.

Sun, J., Chen, Y., Li, M., and Ge, Z. 1998. Role of Antioxidant Enzymes on Ionizing Radiation Resistance. Free Radical Biology & Medicine. 24(4): 586-593.

Mohammed, A. H. M. A, Mohammed, H. I., Zaki, L. M., and Mogazy, A. M. 2012. Pre-exposure To Gamma Rays Alleviates The Harmful Effect Of Salinity On Cowpea Plants. Journal of Stress Physiology & Biochemistry. 8: 199-217.

Hegazi, A. Z., and Hamideldin, N. 2010. The Effect Of Gamma Irradiation On Enhancement Of Growth And Seed Yield Of Okra [Abelmoschus Esculentus (L.) Monech] And Associated Molecular Changes. Journal of Holticulture And Forestry. 2(3): 38-51.

Carpentier, S. C., Panis, B., Vertommen, A., Swennen, R., Sergeant, K., Renaut, J., Laukens, K., Witters, E., Samyn, B., and Devreese, B. 2007. Proteome Analysis Of Non-Model Plants: A Challenging But Powerful Approach. Mass Spectrometry Reviews. 27: 354-377.

Chen, X., Zhang, W., Xie, Y., Lu, W., and Zhang, R. 2007. Comparative Proteomics Of Thylakoid Membrane From A Chlorophyll B-Less Rice Mutant And Its Wild Type. Plant science. 173: 397-407.

Hu, M., Qio, Z, Zhou, P, Xu, L, and Zhang, J. 2012. Proteomic Analysis Of ‘Zaosu’ Pear (Pyrus Bretschneideri Rehd.) And Its Red Skin Bud Mutation. Proteome Science. 10: 1-15.

Zhang, L., Feng, D, Fang, W, Ouyang, H, Luo, Y, Du, T, and Jin, C. 2009. Comparative Proteomic Analysis Of An Aspergillus Fumigatus Mutant Deficient In Glucosidase I (Afcwh41). Microbiology. 155: 2157-2167.




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