EFFECT OF PHOTOPERIOD ON THE GROWTH OF CHLAMYDOMONAS INCERTA AND POLLUTANT REMOVAL
DOI:
https://doi.org/10.11113/mjce.v29.15683Keywords:
Photoperiod, Chlamydomonas incerta, microalgae, POMEAbstract
The key to an economic and healthy algal culture is to optimize the growth conditions. The main objective of this research was to investigate the effects of photoperiod (12:12, 16:8 and 24:0 light: dark cycle) on the growth of indigenous microalgae Chlamydomonas incerta (C. incerta) when cultured in Palm Oil Mill Effluent (POME). POME is nutrient-rich wastewater and one of the highest organic content that could enhance formation of algal bloom. The amount of light intensity and photoperiod has major influence on biomass productivity and photosynthesis process. Microalgae cultures were grown at room temperature and subjected to light source with intensity of ±100 mol m-2 s-1. The growth rate was evaluated based on Optical Density (OD) measured every alternate day during 17 days. Whereas pollutant removal’s ability was determined based on reduction in Chemical Oxygen Demand (COD) of POME. This study revealed that, C. incertaisa mixotrophic microalga because 12:12 light: dark cycle has resulted in the highest biomass concentration (0.786 g L-1) which is eight times the initial concentration and similarly biomass productivity and specific growth rate, 0.04 g L-1d-1 and 0.118 d-1, respectively. The highest pollutants removal was achieved at 12:12 L:D cycle with 70 % COD removal. These result specified that the photoperiod condition has notable impacts on adjusting pollutant removal and producing microalgal biomass. Therefore, the control of photoperiod was proposed as a significant operating parameter in the microalgal wastewater treatment.References
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