EFFECT OF CEMENTATION REAGENTS CONCENTRATIONS ON MICROBIAL CALCITE PRECIPITATION IN RESIDUAL SOIL
DOI:
https://doi.org/10.11113/mjce.v29.15684Keywords:
Residual soil, microorganism, calcite, cementation reagents, soil improvementAbstract
Microbially induced calcite precipitation has been under investigation since early 1990s for its potential application in improving the strength and durability of construction materials such as limestone and cementitious materials. However, excellent results demonstrated by this technique in the studies so far conducted have shown greater potential of exploring its wider applications in geotechnical engineering. This study examines the microbial carbonate precipitations in tropical residual soil via urea hydrolysis. An isolate of urease active strain of Klebsiella pneumoniae UM123 was used to precipitates calcite into the soil with the aim of improving the strength and reducing the hydraulic conductivity of the soil. Cementation reagents concentrations of 0.25, 0.5, 0.75, 1.0 and 1.5M were used to evaluate the strength and hydraulic conductivity of the soil using treatment durations of 24, 48 and 60 hours. Meanwhile, bacteria concentrations of 1.5×104 cfu/ml and 2.9×106 cfu/ml were used to assess the effect of microbial concentrations in the study. The results obtained indicated a general increase in the strength of the treated soil as the bacteria concentrations increases. Likewise, the strength also increases with the increase in reagents concentrations up to 0.5M after which the strength declined. The hydraulic conductivity also decreased by 50% and 65% after 48 hours for 1.5×104cfu/ml and 2.9×106cfu/ml bacteria concentrations. The results so far obtained revealed that the optimum reagent concentration for this particular microorganism for efficient soil improvement is 0.5M.References
Bang, S. C. and Bang, S. S. (2011). KGS Awards Lectures: application of microbiologically induced soil stabilization technique for dust suppression. International Journal of Geo-Engineering. 3(2), 27-37.
Burbank, M. B., Weaver, T. J., Green, T. L., Williams, B. C. and Crawford, R. L. (2011). Precipitation of calcite by indigenous microorganisms to strengthen liquefiable soils. Geomicrobiology Journal. 28(4), 301-312.
Burne, R. A. and Chen, Y.-Y. M. (2000). Bacterial ureases in infectious diseases. Microbes and Infection. 2(5), 533-542.
Castanier, S., Le Metayer-Levrel, G. and Perthuisot, J.-P. (2000). Bacterial roles in the precipitation of carbonate minerals Microbial sediments (pp. 32-39)Springer.
Castanier, S., Le Métayer-Levrel, G. and Perthuisot, J.-P. (1999). Ca-carbonates precipitation and limestone genesis—the microbiogeologist point of view. Sedimentary Geology. 126(1), 9-23.
DeJong, J. T., Fritzges, M. B. and Nüsslein, K. (2006). Microbially induced cementation to control sand response to undrained shear. Journal of Geotechnical and Geoenvironmental Engineering. 132(11), 1381-1392.
DeJong, J. T., Mortensen, B. M., Martinez, B. C. and Nelson, D. C. (2010). Bio-mediated soil improvement. Ecological Engineering. 36(2), 197-210.
Ferris, F., Phoenix, V., Fujita, Y. and Smith, R. (2004). Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 C in artificial groundwater. Geochimica et Cosmochimica Acta. 68(8), 1701-1710.
Ferris, F. G. and Stehmeier, L. G. (1992). Bacteriogenic mineral plugging. Google Patents.
Fujita, Y., Ferris, F. G., Lawson, R. D., Colwell, F. S. and Smith, R. W. (2000). Subscribed content calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiology Journal. 17(4), 305-318.
Fujita, Y., Taylor, J. L., Gresham, T. L., Delwiche, M. E., Colwell, F. S., McLing, T. L., Petzke, L. M. and Smith, R. W. (2008). Stimulation of microbial urea hydrolysis in groundwater to enhance calcite precipitation. Environmental science & technology. 42(8), 3025-3032.
Fujita, Y., Taylor, J. L., Wendt, L. M., Reed, D. W. and Smith, R. W. (2010). Evaluating the potential of native ureolytic microbes to remediate a 90Sr contaminated environment. Environmental science & technology. 44(19), 7652-7658.
Hammes, F., Boon, N., de Villiers, J., Verstraete, W. and Siciliano, S. D. (2003). Strain-specific ureolytic microbial calcium carbonate precipitation. Applied and environmental microbiology. 69(8), 4901-4909.
Ismail, M., Joer, H., Randolph, M. and Meritt, A. (2002). Cementation of porous materials using calcite. Geotechnique. 52(5), 313-324.
Ivanov, V. and Chu, J. (2008). Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Reviews in Environmental Science and Bio/Technology. 7(2), 139-153.
Kavazanjian Jr, E., Iglesias, E. and Karatas, I. (2009). Biopolymer soil stabilization for wind erosion control. Proceedings of the 2009 Proc. 17th Int. Conf. Soil Mech. Geotech. Engng, Alexandria, 881-884.
Konhauser, K. O. (2009). Introduction to geomicrobiology. John Wiley & Sons.
Lioliou, M. G., Paraskeva, C. A., Koutsoukos, P. G. and Payatakes, A. C. (2007). Heterogeneous nucleation and growth of calcium carbonate on calcite and quartz. Journal of colloid and interface science. 308(2), 421-428.
Mitchell, A. C. and Ferris, F. G. (2006). The influence of Bacillus pasteurii on the nucleation and growth of calcium carbonate. Geomicrobiology Journal. 23(3-4), 213-226.
Mitchell, J. K. and Santamarina, J. C. (2005). Biological considerations in geotechnical engineering. Journal of geotechnical and geoenvironmental engineering. 131(10), 1222-1233.
Montoya, B. M. (2012). Bio-mediated soil improvement and the effect of cementation on the behavior, improvement, and performance of sand. University of California, Davis.
Mortensen, B. and DeJong, J. (2011). Strength and stiffness of MICP treated sand subjected to various stress paths. Proceedings of the 2011 Reston, VA: ASCEProceedings of the Geo-Frontiers 2011 conference, March 13-16, 2011, Dallas, Texas| d 20110000: American Society of Civil Engineers,
Nemati, M. and Voordouw, G. (2003). Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzyme and Microbial Technology. 33(5), 635-642.
Okwadha, G. and Li, J. (2010). Optimum conditions for microbial carbonate precipitation. Chemosphere. 81(9), 1143-1148.
Soon, N. W., Lee, L. M., Khun, T. C. and Ling, H. S. (2013). Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE Journal of Civil Engineering. 17(4), 718-728.
Soon, N. W., Lee, L. M., Khun, T. C. and Ling, H. S. (2014). Factors Affecting Improvement in Engineering Properties of Residual Soil through Microbial-Induced Calcite Precipitation. Journal of Geotechnical and Geoenvironmental Engineering. 140(5).
Stocks-Fischer, S., Galinat, J. K. and Bang, S. S. (1999). Microbiological precipitation of CaCO 3. Soil Biology and Biochemistry. 31(11), 1563-1571.
Tai, C. Y. and Chen, P. C. (1995). Nucleation, agglomeration and crystal morphology of calcium carbonate. AIChE Journal. 41(1), 68-77.
van Paassen, L. A., Daza, C. M., Staal, M., Sorokin, D. Y., van der Zon, W. and van Loosdrecht, M. (2010). Potential soil reinforcement by biological denitrification. Ecological Engineering. 36(2), 168-175.
Warren, L. A., Maurice, P. A., Parmar, N. and Ferris, F. G. (2001). Microbially mediated calcium carbonate precipitation: implications for interpreting calcite precipitation and for solid-phase capture of inorganic contaminants. Geomicrobiology Journal. 18(1), 93-115.
Whiffin, V. S. (2004). Microbial CaCO3 precipitation for the production of biocement, Murdoch University.
