EFFECTS OF THIOBENCARB AND BIOAUGMENTATION IN PADDY SOIL AND SEDIMENT SLURRIES ON BACTERIAL COMMUNITY AND THIOBENCARB DEGRADATION
DOI:
https://doi.org/10.11113/aej.v14.22023Keywords:
Thiobencarb, degradation, anaerobic condition, augmentation, bacterial communities.Abstract
Thiobencarb has been extensively applied to control weeds. In this study, research was conducted to investigate thiobencarb degradation in soil and sediment slurries under anaerobic condition whereby we acknowledged degradation rates in this order: sediments from river, soil from paddy field, sediments from mangrove. The augmentation of Dechloromonas sp. Th1, Thauera sp. Th2, and Azoarcus sp. Th3 increased thiobencarb degradation by 24.46±4.1% in soil from paddy fields, 25.03±3.3% in sediment from the river, and 17.58±3.0% in sediment from the mangrove. Moreover, the thiobencarb supplementation and bacterial inoculation resulted in the shifts of bacterial communities in these media. For example, the inoculation of the mixed culture resulted in significant increases in relative abundances of all phyla Dechloromonas, Thauera and Azoarcus in sediment from the river, Dechloromonas and Azoarcus in soil from the paddy field, and Thauera in sediment from the mangrove. α and β-diversity analyses showed that thiobencarb caused significant changes of indigenous bacteria in soil collected from the paddy field and sediment from the mangrove. Besides, the inoculation of the mixed culture reduced the adverse effect of thiobencarb on indigenous bacteria based on α-diversity. This study provides valuable information on roles of bioaugmentation in the degradation, effects of thiobencarb supplementation and bacterial inoculation on native bacterial communities under anaerobic conditions.
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