HIGHLY COMMERCIAL FISHERIES TAWAR FISH: MOLECULAR ANALYSIS DNA MITOCHONDRIAL COI GENE SEQUENCE AND PROXIMATE ANALYSIS FROM MALACCA STRAIT, RIAU
DOI:
https://doi.org/10.11113/jt.v78.8149Keywords:
COI gene, proximate analysis, swim bladder, tawar fishAbstract
Tawar fish is one of fish that live at the Coast of Malacca strait, Riau. Even though they live at sea but the local community has mentioned it as “tawar†fish, which means freshwater fish. Tawar fish is not found in the other place. The most interesting thing of this fish is their dried swim bladder that have an expensive price can reach as much as IDR 15 000 000                 (± USD 1 200) per kg. The local community usually sell them directly to Singapore. The myth has found that this swim bladder is useful for health and could recover diseases although the actual benefit for health has not been well explored. The aim of this research was to investigate molecular approach with PCR amplification COI gene (Cytochrome Oxidase subunit I) and by means proximate analysis on the content muscle and swim bladder. The result showed that tawar fish (FBR_BCL with accession number LC064301) have 92 % sequence similarity with Boesemania microlepsis. While through the proximate analysis, it was found that this species had high protein mainly in swim bladder as much as 36.21 %. Boesemania microlepsis (Bleeker, 1858) have an economic value.
References
Hebert, P. D. N., A. Cywinska, S. L. Ball, and J. R. deWaard. 2003. Biological Identifications through DNA Barcodes. ProcRSocLond. 270: 313–322.
Ivanova, N. V., T. S. Zemlak, R. H. Hanneer, and P. D. N. Hebert. 2007. Barcoding Universal Primer Cocktails for Fish DNA Barcoding. Molecular Ecology Notes. 7(4): 544–548.
Ward, R. D., T. S. Zemlak, B. H. Innes, P. S. Last, and P. D. N. Hebert. 2005. DNA Barcoding Australia's Fish Species. Phil. Trans. R. Soc. 360: 1847–1857.
Parenti, L. R., R. K. Hadiaty, D. Lumbantobing, and F. Herder. 2013. Two New Rice Fishes of The Genus Oryzias (Atherinomorpha: Beloniformes: Adrianichthydae) Augment The Endemic Freshwater Fish Fauna of South Eastern Sulawesi, Indonesia. Copeia. 3: 403–414.
FAO. 1999. The Living Marine Resources of the Western Central Pacific. Vol 3–6. In Carpenter, K. E. and V. H. Niem (eds). Species Identification Guide For Fishery Purposes. Rome: FAO.
Walsh, P. S., A. M. David, and H. Russel. 2013. Biotechniques 30th Anniversary Gem: Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material. Biotechniques. 10(4): 506–513.
Susilowati, R., A. Sabdono, and I. Widowati. 2015. Isolation and Characterization of Bacteria Associated with Brown Algae Sargassum spp. from Panjang Island and Their Antibacterial Activities. In Hady, H., H. Susanto and O. K. Radjasa. Procedia Environmental Sciences. 23: 240–246.
Chun L. P., S. H. Liu, N. L. Chao, F. K. E. Nunoo, H. K. Mok, and W. J. Chen. 2015. A Multi-gene Dataset Reveals a Tropical New World Origin and Early Miocene Diversification of Croakers (Perciformes: Sciaenidae). Molecular Phylogenetics and Evolution. 88: 132–143.
Radjasa, O. K., T. Martens, H. P. Grossart, T. Brinkhoff, A. Sabdono, and M. Simon. 2007. Antagonistic Activity of A Marine Bacterium Pseudoalteromonas luteoviolacea TAB4.2 Associated with Coral Acropora sp. Journal of Biological Sciences. 7(2): 239–246.
Sembiring, A., N. P. D. Pertiwi, A. Mahardini, R. Wulandari, E. M. Kurniasih, A. W. Kuncoro, et al. 2015. DNA Barcoding Reveals Targeted Fisheries for Endangered Sharks in Indonesia. Fisheries Research. 164: 130–134.
Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTALW: Improving The Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position Specific Gap Penalties and Weight Matrix Choice. Nucleic Acids Res. 22: 4673–4680.
Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA 5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution. 28(10): 2731–2739.
AOAC (Association of Official Analytical Chemists). 2000. Official Methods of Analysis of The Association of Official Analytical Chemists. Vol I & II. 17th edition. Arlington: Association of Analytical Chemists.
Haye, P. A., N. I. Segovia, R. Vera, M. A. Gallardo, and C. Gallardo-Escárate. 2012. Authentication of Commercialized Crab-Meat in Chile using DNA Barcoding. Food Control. 25: 239–244.
Hubert, N., R. Hanner, E. Holm, N. E. Mandrak, E. Taylor, M. Burridge, et al. 2008. Identifying Canadian Freshwater Fishes through DNA Barcodes. PLoS One. 3: 2490.
Veneza, I., B. Felipe, J. Oliveira, R. Silva, I. Sampaio, H. Schneider, et al. 2014. A Barcode for The Authentication of Snappers (Lutjanidae) of The Western Atlantic: rDNA 5S or mitochondrial COI? Food Control. 38: 116–123.
Vann, L. S., E. Baran, C. Phen, T. B. Thang. 2006. Biological Reviews of Important Cambodian Fish Species, Base on Fish Base 2004. Volume 2: Pangasius larnaudii; Clarias batrachus; Cirrhinus microlepis; Leptobarbush oevenii; Thynnichthys thynnoides; Trichogaster microlepis; Trichogaster pectoralis; Anabas testudineus; Boesemania microlepis; Oxyeleotris marmorata. Cambodia: World Fish Centre and Inland Fisheries Research and Development Institute.
Takehana, Y., K. Naruse, and M. Sakaizumi. 2005. Molecular Phylogeny of The Medaka Fishes Genus Oryzias (Beloniformes: Adrianichthydae) Based on Nuclear and Mitochondrial DNA Sequences. Molecular Phylogenetic and Evolution. 36: 417–428.
Barbosa, A. J. B., I. Sampaio, H. Schneider, and S. Santos. 2014. Molecular Phylogeny of Weakfish Species of The Stellifer Group (Sciaenidae, Perciformes) of The Western South Atlantic Based on Mitochondrial and Nuclear Data. PLoS ONE. 9: 102250.
Zhang, D. X., and G. M. Hewitt. 1997 Assessment of The Universality and Utility of a Set of Conserved Mitochondrial Primers in Insects. Insect Mol Biol. 6: 143–150.
Knowlton, N., and L. A. Weigt. 1998 New Dates and New Rates for Divergence Across The Isthmus of Panama. Proc. R. Soc. Lond. 265: 2257–2263.
Ravichandran, S., K. Kumaravel, G. Rameshkumar, and T. T. Ajithkumar. 2010. Antimicrobial Peptides from Marine Fishes. Res. J. Immunol. 3: 146–156.
Louka, N., F. Juhel, V. Fazilleau, and P. Loonis. 2004. A Novel Colorimetry Analysis used to Compare Different Drying Fish Process. J. Food. Control. 15: 327–334.
Sutharshiny, S., and Sivashanthini. 2011. Proximate Composition of Three Species of Scomberoides Fish from Sri Lankan Waters. Asian Journal of Clinical Nutrition. 3(3): 103–111.
Gokhan, B., and K. Hikmet. 2011. Seasonal Changes in Proximate Composition of Some Fish Species from The Black Sea. Turk. J. Fish. Aquat. Sci. 11: 1–5.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.