MOLECULAR CHARACTERIZATION OF Dunaliella salina AND Chlorella vulgaris FUSANT USING 18SrDNA GENE
DOI:
https://doi.org/10.11113/jt.v78.8155Keywords:
Chlorellla, Dunaliella, protoplast fusion, 18SrDNAAbstract
Protoplast fusion was found to be an efficient method in improving carotenoid production from fusant of carotenogenic microalgae D. salina and C. vulgaris. Molecular characterization is needed for identifying the dominant parental genome in the fusant using ribosomal DNA sequences. The research was carried out by analyzing the gene encodes for 18S rDNA of fusant and determining relationship of fusant with D. salina and C. vulgaris species from GenBank. Quantitative analysis showed that C. vulgaris was not remarkably dominant in fusant with 84 % similarity compare to D. salina with 82 % similarity. The result indicated that the fusant gained both character from their parents.
References
Lee, Y. K. and H. M. Tan. 1988. Interphylum Protoplast Fusion and Genetic Recombination of the Algae Porphyridium cruentum and Dunaliella spp. Journal of General Microbiology. 134: 635–641.
Lu, Y., R. Kong, and L. Hu. 2012. Preparation Protoplasts from Chlorella protothecoides. World Journal of Microbiology Biotechnology. 28(4): 1827–1830.
Santiago, C.M. 1982. Protoplast Fusion A New Tecnique for Genetic Manipulation and Breeding of Industrial Microorganisme. I.C. Biotech. 5: 435–440
Pulz, O. and W. Gross. 2004. Valuable Products from Biotechnology of Microalgae. Applied Microbiology Biotechnology. 65: 635–648.
Borowitzka, M. A. and L. J. Borowitzka. 1988. Dunaliella. In Borowitzka, M. A. (ed.). Micro-Algal Biotechnology. Cambridge: Cambridge University Press
Kusumaningrum, H. P. and M. Zainuri. 2014. Optimization and Stability of Total Pigments Production of Fusan from Protoplast Fusion of Microalgae Dunaliella and Chlorella in vivo. Attempts on Production of Sustainable Aquaculture Natural Food. International Journal of Marine and Aquatic Resource Conservation and Co-existance. 1(1): 1–5
Guedes, A.C. and F. X. Malcata. 2012: Nutritional Value and Uses of Microalgae in Aquaculture. In Muchlisin, Z. A. (ed). Aquaculture. Rijeka: InTech.
Guedes, A. C., H. M. Amaro, and F. X. Malcata, 2011. Microalgae as Sources of Carotenoids. Marine Drugs. 9: 625–644.
Panaiotov S., Y. Evstatieva, S. Ilieva, V. Levterova, N. Brankova, D. Nikolova, et al. 2009. Quantitative Assesment of The Dominant Genome in Fusant Cultures. Biotechnology and Biotechnological Equipment, supp.1. SE. 23: 892ï€895.
Zainuri, M., E. Kusdiyantini, Widjanarko, J. Soedarsono, and T. Yuwono, 2003. Preliminary Study on the Use of Yeast Phaffia rhodozyma as Pigment Source on The Growth of Tiger Shrimp (Penaeus monodon Fabricius). Indonesian Journal of Marine Sciences. 8(1): 47–52
Boonyaratpalin, M., S. Thongrod, K. Supamattaya, G. Britton, and L. E. Schlipalius. 2001. Effects of ï¢-carotene Source, Dunaliella salina, and Astaxanthin on Pigmentation, Growth, Survival and Health of Penaeus monodon. Aquaculture Research. 32: 182–190.
Kusumaningrum, H. P. and M. Zainuri. Detection of Bacteria and Fungi Associated with Penaeus monodon Postlarvae Mortality. International Journal Procedia Environmental Sciences. 23: 329–337.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
