EFFECT OF DIFFERENT CONCENTRATION SALT AND TRYPSIN ON THE PHYSICOCHEMICAL PROPERTIES OF FISH SAUCE MADE FROM SEA CATFISH (Arius sp.) VISCERA
DOI:
https://doi.org/10.11113/jt.v78.8188Keywords:
Fish sauce, physicochemical properties, salt, sea catfish viscera, trypsinAbstract
Fish sauce is a fermented product for seasoning that is popular in Asian country. One of the problems to produce this product is a long fermentation time up to 1 yr to 2 yr. Addition of trypsin as proteolytic enzyme and decreasing salt concentration is done to accelerate the fermentation time. This research examined the effect of trypsin addition and different salt concentrations (15 %) and the physicochemical properties of fish sauce made from sea cat fish (Arius sp.) after 45 d of fermentation. The result showed that  the higher salt and enzyme concentrations gave higher yield (48.54 %), color intensity (33.6 %) and salt content          (24.78 %) but also gave lower TVBN (26.1 mgN per 100 g), TMA (11.14 mgN per 100 g), Ammonia (12.87 mgN per 100 g) and pH level (5.4). This result could meet the standard of fish sauce from codex on pH and salt content.
References
Shahidi, F. and J. Y. V. A. Kamil. 2001. Enzymes from Fish and Aquatic Invertebrates and Their Application in the Food Industry. Food Science and Technology. 12: 435–464.
Simpson, B. K. 2000. Digestive Proteinases from Marine Animals. In Haard, N. F., Simpson, B. K. (eds.). Seafood Enzymes Utilization and Influence on Postharvest Seafood Quality. New York: Mercel Dekker. 531–540.
Gildberg, A. and C. Thongthai. 2001. The Effect of Reduced Salt Content and Addition of Halophilic Lactic Bacteria on Quality and Composition of Fish Sauce Made from Sprat. Aquat. Food Product Technology. 10: 77–88.
Klomklao, S., S. Benjakul, and W. Visessanguan. 2004. Comparative Studies on Proteolytic Activity of Spleen Extracts from Three Tuna Species Commonly Used in Thailand. Journal of Food Biochemistry. 28: 355–372.
Saisithi, P. 1994. Traditional Fermented Fish: Fish Sauce Production. In Martin, A. M. (ed.).Fisheries Processing: Biotechnological Applications. Chapman and Hall: London, UK. 111–131.
Lopetcharat, K., Y. J. Choi, J. W. Park, and M. A. Daeschel. 2001. Fish Sauce Products and Manufacturing: A Review. Food Reviews International. 17: 65–68.
Fukami, K., Y. Funatsu, K. Kawasaki, and S. Watabe. 2004. Improvement of Fish Sauce Odor by Treatment With Bacteria Isolate From the Fish Sauce Mush (Moromi) Made from Frigate Mackerel. J. Food Sci. 69: 45–49.
Baird-Parker, T. C., 2000. In Lund, B. M. and T. C. Baird-Parker (Eds.). The Production of Microbiologically Safe and Stable Foods. Aspen Publishers Inc., Gaithersburg, MD., USA.
Amos, B. 2007. Analysis of Quality Deterioration at Critical Steps/Points in Fish Handling in Uganda and Iceland and Suggestions for Improvement. United Nations University, Uganda. [Online]. From: http://www.unuftp.is/ static/fellows/document/amos06prf.pdf. [Accessed on 15 Desember 2015].
Morioka, K., S. Fujii, Y. Iton, L. Chengchu, and A. Obatake. 1999. Recovery of Amino Acid from Protein in the Head and Viscera of Frigate Mackerel by Autolysis. Fish. Sci. 65: 588–591.
Kim, S., Y. Jeon, H. Byeun, Y. Kim, and C. Lee. 1997. Enzymatic Recovery of Cod Frame Proteins with Crude Proteinase from Tuna Pyloric Caeca. Fish Sci. 63: 421–427.
Gildberg, A. 2001. Utilization of Male Arctic Capelin and Atlantic Cod Intestine for Fish Sauce Production–Evaluation of Fermentation Conditions. Bioresource Technology. 76:119-123.
Klomklao, S., S. Benjakul, W. Visessanguan, H. Kishimura, and B. K. Simpson 2006. Effects of the Addition of Spleen of Skipjack Tuna (Katsuwonus pelamis) on the Liquefaction and Characteristics of Fish Sauce Made from Sardine (Sardinella gibbosa). Food Chemistry. 98: 440–452.
Amano, K. 1962. The Influence of Fermentation on the Nutritive Value of Fish with Special Reference to Fermented Fish Products of South-East Asia. In Heen, E., Kreuzer, R. (eds.). Fish in Nutrition. Proceedings of the FAO Congress, Washington DC, Fishing News Books: London. 180–197.
Orejana, F. M. and J. Liston. 1981. Agents of Proteolysis and Its Inhibition in Patis (Fish Sauce) Fermentation. J. Food Sci. 47: 198–203.
Gildberg, A., and S. Xian-Quan. 1994. Recovery of Tryptic Enzymes from Fish Sauce. Process Biochem. 29: 151–155.
Xu, W., G. Yu, C. Xue, Y. Xue, and Y. Ren. 2008. Biochemical Changes Associated with Fast Fermentation of Squid Processing By-Product for Low Salt Fish Sauce. Food Chemistry. 107: 1597–1604.
Conway, E. J. and A. Byrne. 1936. An Absorption Apparatus for the Microdetermination of Certain Volatile Substances: The Micro-determination of Ammonia. J. Biochem. 27: 419–429.
Yunizal, J. T., N. Murtini., B. Dolaria., Purdiwoto, Abdulrokhim, and Carkipan. 1998. Prosedur Analisa Kimiawi Ikan dan Produk Olahan Hasil-Hasil Perikanan [Chemical Analysis Procedures in Fish and Fisheries Products]. Jakarta: Balai Penelitian Perikanan Laut Slipi. [Bahasa Indonesia].
Gomez, A. K. and A. A. Gomez. 1995. Prosedur Statistik untuk Penelitian Pertanian [Statistic Procedure for Agricultural Research]. Jakarta: UI–Press. [Bahasa Indonesia].
Ames, J. M. 1992. The Maillard Reaction. In Hudson, B. J. F. (ed.). Biochemistry of Food Proteins. London: Elsevier Applied science. 99–153.
Kawashima, K., and H. Yamanaka. 1996. Free Amino Acids Responsible for the Browning of Cooked Scallop Adductor Muscle. Fisheries Science. 62: 293–296.
Ishida, M., S. Niizelei, and F. Nagayama. 1994. Thermostable Proteinase in Salted Anchovy Muscle. Food Science. 59: 781–785.
Hebard, C. E., G. J. Flick, and R. E. Martin. 1982. Occurrence and Significance of Trimethylamine Oxide and Its Derivatives in Fish and Shellfish. In Martin, R. E., G. J. Flick, C. E. Hebard, and D. R. Ward (eds.). Chemistry and Biochemistry of Marine Food Products. Westport: AVI Publishing: Westport.
Dissaraphong, S., S. Benjakul, and W. Visessanguan. 2006. The Influence of Storage Conditions of Tuna Viscera before Fermentation in the Chemical, Physical and Microbiological Changes in Fish Sauce during Fermentation. Bioresource Technology. 97: 2032–2040.
Connell, J. J. 1975. Control of Fish Quality. Surrey: Fishing News.
Gram, L. and H. H. Huss. 1996. Microbial Spoilage of Fish and Fish Products. Inter. J. Food Microbiol. 33: 121–137.
Sanceda, N. G., R. Suzuki, M. Ohashi, and T. Kurata. 1999. Histamine Behavior during the Fermentation Process in the Manufacture of Fish Sauce. J. Agric. Food Chem. 47: 3596–3600.
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