DNA BARCODING FOR IDENTIFICATION OF PROCESSED TUNA FISH IN INDONESIAN MARKET
DOI:
https://doi.org/10.11113/jt.v78.8190Keywords:
Mislabeling, PCR, tunaAbstract
DNA barcoding is a molecular technique to characterize species organism using a short DNA sequence. Recently, it becomes useful tool to detect seafood mislabeling and species substitution. Cytochrome b is one of the mitochondrial gene used in DNA barcoding. In order to face the regulation of AFTA (ASEAN Free Trade Area), the accurate method to detect the fish species and its products is needed in order to avoid the fraudulent in Indonesia. Thus, an attempt was carried out to identify authentication for tuna’s products (sushi, fish ball, meat floss, and canned tuna). The samples were collected from, Bogor, West Java, Indonesia. DNA was isolated according to the manufacturer’s protocol. The amplification of DNA by PCR was carried out, then the direct sequence was performed. In the present study, DNA barcoding of tuna’s product using cytochrome b were elucidated. The amplification of DNA by PCR was successfully obtained from tuna’s sample except one of canned tuna (K3). It showed that one of the canned tuna as in the label did not contain tuna. It indicated that there was an economic fraud for one of canned tuna.
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