DECOLOURISATION OF REACTIVE BLACK 5 BY KLEBSIELLA PNEUMONIAE ISOLATED FROM ANTARCTICA SEAWATER
DOI:
https://doi.org/10.11113/jt.v79.10811Keywords:
Azo dye, Reactive Black 5, decolourisation, Klebsiella pneumonia, Antarctica seawaterAbstract
The discharge of highly coloured azo dyes effluent has caused serious environmental damages. In this study, bacteria isolated from Antarctica seawater were screened for their ability to decolourise azo dye Reactive Black 5 (RB5). The selected bacterium was further investigated to study its ability to decolourise RB5. The best bacteria from Antarctica seawater that had the ability to decolourise RB5 was identified using 16S rDNA sequence analysis and revealed that the bacteria 15C shared 99% homology to Klebsiella pneumoniae. Selection of the most effective bacteria was followed by its acclimatisation to decolourise higher concentrations of RB5 by growing it in successively higher concentrations of RB5. Following that, optimization of RB5 decolourisation by the selected bacteria was performed using one factor at time (OFAT) including concentration of dye, pH and temperature. The results obtained indicated the optimal condition for decolourisation of RB5 using this bacterium was at pH 10 and 37°C in 70 mg/L RB5 with 98% decolourisation within 24 h under facultative anaerobic treatment. Besides that, 70% of COD removal was achieved after 96 h of sequential anaerobic and aerobic treatment of RB5. In addition, FTIR and HPLC were used to analyze the metabolite of RB5 decolourisation. Products of RB5 decolourisation was confirmed by the presence of sulphanilic acid in HPLC analyses and the changes observed in the functional groups of the FTIR spectrum suggesting the possibility of RB5 degradation. Ammonia test and carbon dioxide test showed higher concentration of ammonia and carbon dioxide that indicates the mineralisation of product after treatment.
References
Sedigheh Asad, Seyed Mohammad Mehdi Dastgheib, Mohammad Ali Amoozegar. 2014. Optimization for Decolorization of Azo Dye Remazol Black B by a Halomonas Strain Using the Taguchi Approach. Progress in Biological Sciences. 4(1): 33-42.
Siti Aishah Mohd Hanim. 2012. Optimization of Decolourization of Reactive Black 5 using Brevibacillus Panacihumi. Master Thesis. Universiti Teknologi
Shertate, R. S. and Thorat, P. R. 2013. Decolorization and Biodegradation of Textile Azo Dye Sunset Yellow FCF by Acclimatized Marine Bacteria. Indian Streams Research Journal. 3(2): 2230-7850.
Shahid Mahmood, Muhammad Arshad, Azeem Khalid, Zilli Huma Nazli and Tariq Mahmood. 2011. Isolation and Screening of Azo Dye Decolorizing Bacterial Isolates from Dye-contaminated Textile Wastewater. Soil Science Society of Pakistan. 30(1): 7-12.
N. Mahesh Kumar and D. Saravanan. 2015. Isolation of Dye Degrading Bacteria from Textile Effluent. Journal of Chemical and Pharmaceutical Research. 7(3): 2214-2218.
Vijaykumar, M.H., P.A. Vaishampayan, Y.S. Shouche and T. B. Karegoudar. 2007. Decolourization of Naphthalene-Containing Sulfonated Azo Dyes by Kerstersia sp. Strain VKY1. Enzyme and Microbial Technology. 40: 204-211.
Cheng, K. C., Wu, J. Y., Liou, D. J. and Hwang, S. C. J. 2003. Decolorization of the Textile Azo Dyes by Newly Isolated Bacterial Strains. J. Biotechnol. 101: 57-68.
Puvaneswari, N., Muthukrishnan, J. and Gunasekaran, P. 2006. Toxicity Assessment and Microbial Degradation of Azo Dyes. Indian Journal of Experimental Biology. 44: 618-626.
Hildenbrand, S., Schmahl, F. W. and Wodarz R. 1999. Azo Dyes and Carcinogenic Aromatic Amines in Cell Culture. Int Arch Occup Environ Health. 72: M52.
Gomez, V., Larrenchi, M. S. and Callao, M. P. 2007. Kinetic and Adsorption Study of Acid Dye Removal Using Activated Carbon. Chemosphere. 69: 1151-1158.
G. Donmez. 2002. Bioaccumulation of The Reactive Textile Dyes by Candida Tropicalis Growing in Molasses Medium. Enzyme Microb. Technol. 30: 363-366.
I.M Banat. P. Nigam, D. Singh, R. Marchant. 1996. Microbial Decolourisation of Textile Dye Containing Efflunts. A Review, Bioresour. Technol. 58: 217-227.
Y.Fu, T. Viraghavaan. 2001. Fungal Decolorization of Dye Wastewaters: A Review. Bioresour. Technol. 79: 251-262.
Z. Aksu. 2005. Application of Biosorption for the Removal of Organic Pollutants. A Review. Process Biochem. 40: 997-1026.
Pandey, A., P. Singh and L. Lyengar. 2007. Bacterial Decolourisation and Degradation of Zo Dyes. International Biodeterioration and Biodegradation. 59: 73-84.
Hao, J. J., F. Q. Song, F. Huang, C. L. Yang, Z. J. Zhang, Y. Zheng and X. J. Tian. 2007. Production of Laccase by a Newly Isolated Deuteromycete Fungus Pestalotiopsis Sp. and Its Decolorization of Azo Dye. Journal of Industrial Microbiology and Biotechnology. 34: 233-240.
Khalid, A., M. Arshad and D. E. Crowley. 2008. Accelerated Decolorization of Structurally Different Azo Dyes by Newly Isolated Bacterial Strains. Applied Microbiology and Biotechnology. 78: 361-369.
Hassan, M. M., Alam, M. Z. and Anwar, M. N. 2013. Biodegradation of Textile Azo Dyes by Bacteria Isolated from Dyeing Industry Effluent. Int. Res. J. Biological Sci. 2(8): 2278-3202.
Cırık, Kevser, and Özer Çınar. 2010. Parameters Affecting Anaerobic Color Removal of Textile Wastewaters. 2nd International Symposium on Sustainable Development, June 8-9 2010, Sarajevo. 571-578.
Dafale, N., L. Agrawal, A. Kapley, S. Meshram, H. Purohit, S. Wate. 2010. Selection of Indicator Bacteria based on Screening of 16S rDNA Metagenomic Library from a Two-Stage Anoxic–oxic Bioreactor System Degrading Azo Dyes. Bioresource Technology. 101: 476-484.
Kapdan, K. I., Tekol, M. and Sengul, F. 2003. Decolorization of Simulated Textile Wastewater in an Anaerobic Aerobic Sequential Treatment System. Process Biochem. 38: 1031-7.
Ilgi Karapinar Kapdan. 2005. Kinetic Analysis of Dyestuff and COD Removal from Synthetic Wastewater in an Anaerobic Packed Column Reactor. Process Biochemistry. 40: 2545-2550.
Chang, J. S. and Kuo, T. S. 2000. Kinetics of Bacterial Decolorization of Azo Dye with Escherichia coli. Bioresource Technology. 75(2): 107-111.
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