• Nurazeerah Khamis Department of Bio and Natural Resources Technology, Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, Malaysia https://orcid.org/0000-0001-6619-1741
  • Ainihayati Abdul Rahim ᵃDepartment of Bio and Natural Resources Technology, Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jbeli, Kelantan, Malaysia ᵇAdvanced Industrial Biotechnology Cluster, Universiti Malaysia Kelantan, Malaysia
  • Noor Azlina Ibrahim ᵃDepartment of Bio and Natural Resources Technology, Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jbeli, Kelantan, Malaysia ᵇAdvanced Industrial Biotechnology Cluster, Universiti Malaysia Kelantan, Malaysia
  • Khomaizon Abdul Kadir Pahirul Zaman Faculty of Agro Based Industry, Universiti Malaysia Kelantan, Jeli Campus, 17600 Jeli, Kelantan, Malaysia




Biosurfactants, emulsification, hydrocarbon, recovery, surface tension


Surface-active agents or surfactants are amphiphilic compounds derived naturally or chemically synthesized. Natural surfactants or so-called biosurfactants can be obtained from bacteria, yeasts, and fungi as well as plants. Due to their hydrophilic and hydrophobic molecules, biosurfactants offer the ability to reduce surface or interfacial tension and create microemulsion between aqueous and organic phases. Many studies have suggested biosurfactants as a better substitution to chemical surfactants since they are more environment friendly, biocompatible, and has lower toxicity. This review article is provided to introduce some general information regarding microbial-derived biosurfactants including five main classes of biosurfactants and several distinctive properties that confer them as promising surface-active agents. Due to their diverse molecular structures, biosurfactants have been applied in various applications. Thus, several common biosurfactant recovery methods are discussed and examples of biosurfactant applications in hydrocarbon removal as well as in industrial processing are highlighted.


Campos, J. M., Stamford, T. L. M., Sarubbo, L. A., de Luna, J. M., Rufino, R. D., and Banat, I. M. 2013. Microbial Biosurfactants as Additives for Food Industries. Biotechnology Progress. 29(5): 1097-1108.

Lamichhane, S., Krishna, K. C. B., and Sarukkalige, R. 2017. Surfactant-enhanced Remediation of Polycyclic Aromatic Hydrocarbons: A Review. Journal of Environmental Management. 199: 46-61.

Reis, R. S., Pacheco, G. J., Pereira, A. G., and Freire, D. M. G. 2013. Biosurfactants: Production and Applications. R. Chamy, F. Rosenkrantz (Eds.). Biodegrad. - Life Sci., InTechOpen, London. 31-61.

Vecino, X., Cruz, J. M., Moldes, A. B., and Rodrigues, L. R. 2017. Biosurfactants in Cosmetic Formulations: Trends and Challenges. Critical Reviews in Biotechnology. 37(7): 911-923.

Das, P., Mukherjee, S., Sivapathasekaran, C., and Sen, R. 2010. Microbial Surfactants of Marine Origin: Potentials and Prospects. Sen (Ed.). Biosurfactants Adv. Exp. Med. Biol., Landes Bioscience, Texaspp. 88-101.

Hirata, Y., Ryu, M., Oda, Y., Igarashi, K., Nagatsuka, A., Furuta, T., et al. 2009. Novel Characteristics of Sophorolipids, Yeast Glycolipid Biosurfactants, as Biodegradable Low-foaming Surfactants. Journal of Bioscience and Bioengineering. 108(2): 142-146.

Kubicki, S., Bollinger, A., Katzke, N., Jaeger, K. E., Loeschcke, A., and Thies, S. 2019. Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications. Marine Drugs. 17(7): 1-30.

Johnson, P., Pinfield, V. J., Starov, V., and Trybala, A. 2021. Effect of Synthetic Surfactants on the Environment and the Potential for Substitution by Biosurfactants. Advances in Colloid and Interface Science. 288: 102340.

Shekhar, S. and Sundaramanickam, Arumugam Balasubramaniam, T. 2014. Biosurfactant Producing Microbes and Their Potential Applications: A Review. Environmental Science and Technology. 45(14): 1522-1554.

Silva, R. C. F. S., Almeida, D. G., Rufino, R. D., Luna, J. M., Santos, V. A., and Sarubbo, L.. 2014. Applications of Biosurfactants in the Petroleum Industry and the Remediation of Oil Spills. International Journal of Molecular Sciences. 15(7): 12523-12542.

Nurfarahin, A. H., Mohamed, M. S., and Phang, L. Y. 2018. Culture Medium Development for Microbial-derived Surfactants Production—An Overview. Molecules. 23(5): 1-26.

Nitschke, M. and Costa, S. G. V. A. O. 2007. Biosurfactants in Food Industry. Trends in Food Science and Technology. 18(5): 252-259.

Geys, R., Soetaert, W., and Bogaert, I. V. 2014. Biotechnological Opportunities in Biosurfactant Production. Current Opinion in Biotechnology. 30: 66-72.

Marchant, R. and Banat, I. M. 2012. Biosurfactants: A Sustainable Replacement for Chemical Surfactants? Biotechnology Letters. 34(9): 1597-1605.

Liu, J. F., Mbadinga, S. M., Yang, S. Z., Gu, J. D., and Mu, B. Z. 2015. Chemical Structure, Property and Potential Applications of Biosurfactants Produced by Bacillus subtilis in Petroleum Recovery and Spill Mitigation. International Journal of Molecular Sciences. 16(3): 4814-4837.

Vijayakumar, S. and Saravanan, V. 2015. Biosurfactants-types, Sources and Applications. Research Journal of Microbiology. 10(5): 181-192.

Chen, W. C., Juang, R. S., and Wei, Y. H. 2015. Applications of a lipopeptide Biosurfactant, Surfactin, Produced by Microorganisms. Biochemical Engineering Journal. 103 (November): 158-169.

Fracchia, L., Cavallo, M., Martinotti, M. G., and Banat, I. M. 2012. Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials. D.N. Ghista (Ed.). Biomed. Sci. Eng. Technol. InTechOpen, Crotiapp. 325-370.

Sarwar, A., Brader, G., Corretto, E., Aleti, G., Abaidullah, M., Sessitsch, A., et al. 2018. Qualitative Analysis of Biosurfactants from Bacillus species Exhibiting Antifungal Activity. PLoS ONE. 13(6): 1-15.

Rodrigues, L., Banat, I. M., Teixeira, J., and Oliveira, R. 2006. Biosurfactants: Potential Applications in Medicine. Journal of Antimicrobial Chemotherapy. 57(4): 609-618.

Karlapudi, A. P., Venkateswarulu, T. C. Tammineedi, J., Kanumuri, L., Ravuru, B. K., Dirisala, V. R., and Kodali, V. P. 2018. Role of Biosurfactants in Bioremediation of Oil Pollution-A Review. Petroleum.

Santos, D. K. F., Rufino, R. D., Luna, J. M., Santos, V. A., and Sarubbo, L. A. 2016. Biosurfactants: Multifunctional Biomolecules of the 21st Century. International Journal of Molecular Sciences. 17(3): 1-31.

Uzoigwe, C., Burgess, J. G., Ennis, C. J., and Rahman, P. K. S. M. 2015. Bioemulsifiers are not Biosurfactants and Require Different Screening Approaches. Frontiers in Microbiology. 6: 1-6.

Satpute, S. K., Bhuyan, S. S., Pardesi, K. R., Mujumdar, S. S., Dhakephalkar, P. K., Shete, A. M., et al. 2010. Molecular Genetics of Biosurfactant Synthesis in Microorganisms. R. Sen (Ed.). Biosurfactants Adv. Exp. Med. Biol., Landes Bioscience, Texaspp. 14-41.

Mazaheri, A. M. and Tabatabaee, M. S. 2010. Biosurfactants and Their Use in Upgrading Petroleum Vacuum Distillation Residue: A Review. International Journal of Environmental Research. 4(4): 549-572.

Md, F. 2012. Biosurfactant: Production and Application. Journal of Petroleum & Environmental Biotechnology. 03 (04): 1-5.

Kaczorek, E., Pacholak, A., Zdarta, A., and Smułek, W. 2018. The Impact of Biosurfactants on Microbial Cell Properties Leading to Hydrocarbon Bioavailability Increase. Colloids and Interfaces. 2(3): 35.

Shete, A. M., Wadhawa, G., Banat, I. M., and Chopade, B. A. 2006. Mapping of Patents on Bioemulsifier and Biosurfactant: A Review. Journal of Scientific and Industrial Research. 65(2): 91-115.

Wen, Y., Cheng, H., Lu, L.-J., Liu, J., Feng, Y., Guan, W., et al. 2010. Analysis of Biological Demulsification Process of Water-in-oil Emulsion by Alcaligenes sp. S-XJ-1. Bioresource Technology. 101(21): 8315-8322.

Singh, P. and Cameotra, S. S. 2004, Potential Applications of Microbial Surfactants in Biomedical Sciences. Trends in Biotechnology. 22(3): 142-146.

da Rocha Junior, R. B., Meira, H. M., Almeida, D. G., Rufino, R. D., Luna, J. M., Santos, V. A., et al. 2019. Application of a Low-cost Biosurfactant in Heavy Metal Remediation Processes. Biodegradation. 30(4): 215-233.

Nogueira Felix, A. K., Martins, J. J. L., Lima Almeida, J. G., Giro, M. E. A., Cavalcante, K. F., Maciel Melo, V. M., et al. 2019. Purification and Characterization of a Biosurfactant Produced by Bacillus subtilis in Cashew Apple Juice and Its Application in the Remediation of Oil-contaminated Soil. Colloids and Surfaces B: Biointerfaces. 175(November 2018): 256-263.

Dehghan-Noudeh, G., Housaindokht, M., and Bazzaz, B. S. F. 2005. Isolation, Characterization, and Investigation of Surface and Hemolytic Activities of a Lipopeptide Biosurfactant Produced by Bacillus Subtilis ATCC 6633. Journal of Microbiology. 43(3): 272-276.

Fei, D., Zhou, G. W., Yu, Z. Q., Gang, H. Z., Liu, J. F., Yang, S. Z., et al. 2019. Low-Toxic and Nonirritant Biosurfactant Surfactin and its Performances in Detergent Formulations. Journal of Surfactants and Detergents. 23(1): 109-118.

Abha, S. and Singh, C. S. 2012. Hydrocarbon Pollution: Effects on Living Organisms, Remediation of Contaminated Environments, and Effects of Heavy Metals Co-Contamination on Bioremediation. L. Romeo-Zerón (Ed.). Introd. to Enhanc. Oil Recover. Process. Bioremediation Oil-Contaminated Sites, InTech, Croatiapp. 185-206.

Sukirta, T. H. and Usharani, M. V. 2013. Production and Qualitative Analysis of Biosurfactant and Biodegradation of the Organophosphate by Nocardia mediterranie. Journal of Bioremediation & Biodegradation. 04(06): 4-11.

Singh, A. K., Rautela, R., and Cameotra, S. S. 2014. Substrate Dependent In Vitro Antifungal Activity of Bacillus sp strain AR2. Microbial Cell Factories. 13(1): 1-11.

Ehinmitola, E. O., Aransiola, E. F., and Adeagbo, O. P. 2018. Comparative Study of Various Carbon Sources on Rhamnolipid Production. South African Journal of Chemical Engineering. 26: 42-48.

Feng, J. Q., Gang, H. Z., Li, D. S., Liu, J. F., Yang, S. Z., and Mu, B. Z. 2019. Characterization of Biosurfactant Lipopeptide and Its Performance Evaluation for Oil-spill Remediation. RSC Advances. 9(17): 9629-9632.

Janek, T., Krasowska, A., Czyznikowska, Z., and Lukaszewicz, M. 2018. Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach. Frontiers in Microbiology. 9: 1-14.

Sabturani, N., Latif, J., Radiman, S., and Hamzah, A. 2016. Spectroscopic Analysis of Rhamnolipid Produced by Pseudomonas Aeruginosa UKMP14T. Malaysian Journal of Analytical Sciences. 20(1): 31-43.

Shah, M. U. H., Sivapragasam, M., Moniruzzaman, M., and Yusup, S. 2016. A Comparison of Recovery Methods of Rhamnolipids Produced by Pseudomonas aeruginosa. Procedia Engineering. 148: 494-500.

Weber, A. and Zeiner, T. 2015. Purification of Biosurfactants. F. V.-S. Kosaric, N. (Ed.). Biosurfactants, CRC Press. 129-152.

Invally, K., Sancheti, A., and Ju, L.. 2019. A New Approach for Downstream Purification of Rhamnolipid Biosurfactants. Food and Bioproducts Processing. 114: 122-131.

Inès, M., Dhouha, G., and Inès, M. & Dhouha, G. 2015. Lipopeptide Surfactants: Production, Recovery and Pore Forming Capacity. Peptides. 71: 100-112.

Varjani, S. J. and Upasani, V. N. 2017. Critical Review on Biosurfactant Analysis, Purification and Characterization Using Rhamnolipid as a Model Biosurfactant. Bioresource Technology. 232: 389-397.

Satpute, S. K., Banpurkar, A. G., Dhakephalkar, P. K., Banat, I. M., and Chopade, B. A. 2010. Methods for investigating Biosurfactants and Bioemulsifiers : A Review. Critical Reviews in Biotechnology. 30(2): 127-144.

You, Z., Xu, H., Zhang, S., Kim, H., Chiang, P. C., Yun, W., et al. 2018. Comparison of Petroleum Hydrocarbons Degradation by Klebsiella Pneumoniae and Pseudomonas aeruginosa. Applied Sciences. 8(12): 1-19.

Abdul Razak, N. N., Abdullah, N., Awang Biak, D. R., and Mohd Yatim, A. R. 2015. Solvent-less Approach for the Recovery of Palm-based Sophorolipids Biosurfactant Via Salting-out Method. Journal of Oil Palm Research. 27(2): 181-189.

Banat, I. M., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti, G., Fracchia, L., et al. 2010. Microbial Biosurfactants Production, Applications and Future Potential. Applied Microbiology and Biotechnology. 87(2): 427-444.

Najmi, Z., Ebrahimipour, G., Franzetti, A., and Banat, I. M. 2018. In situ Downstream Strategies for Cost-effective Bio/surfactant Recovery. Biotechnology and Applied Biochemistry. 65(4): 523-532.

Bages-Estopa, S., White, D. A., Winterburn, J. B., Webb, C., and Martin, P. J. 2018. Production and Separation of a Trehalolipid Biosurfactant. Biochemical Engineering Journal. 139: 85-94.

Mulligan, C. N. and Gibbsb, B. F. 1990. Recovery of Biosurfactants by Ultrafiltration. Journal of Chemical Technology and Biotechnology. 47: 23-29.

Wahib, Z. M., Mahmood, N. N., and Khudhaier, S. R. 2020. Extraction of Biosurfactant from Pseudomonas aeruginosa and its Effects on Some Pathogenic Bacteria. 20(2): 6700-6704.

Zhang, S., Wu, W., Li, D., and Zheng, Q. 2015. Separation and Purification of Six Biosurfactant Rhamnolipids by High-speed Countercurrent Chromatography Utilizing Novel Solvent Selection Method. Separation Science and Technology (Philadelphia). 51(4): 673-680.

Sonawdekar, S. 2012. Bioremediation : A Boon to Hydrocarbon Degradation. International Journal of Environmental Sciences. 2(4): 2408-2424.

Bustamante, M., Durán, N., and Diez, M. C. 2012. Biosurfactants are Useful Tools for the Bioremediation of Contaminated Soil: A Review. Journal of Soil Science and Plant Nutrition. 12(4): 667-687.

Maletic, S., Dalmacija, B., and Roncevic, S. 2013. Petroleum Hydrocarbon Biodegradability in Soil – Implications for Bioremediation. V. Kutcherov (Ed.). Hydrocarbon, IntechOpen. 43-64.

Souza, E. C., Vessoni-Penna, T. C., and Oliveira, R. P. S. 2014. Biosurfactant-enhanced Hydrocarbon Bioremediation: An Overview. International Biodeterioration and Biodegradation. 89: 88-94.

Franzetti, A., Bestetti, G., Caredda, P., La Colla, P., and Tamburini, E. 2008. Surface-active Compounds and their Role in the Access to Hydrocarbons in Gordonia Strains. FEMS Microbiology Ecology. 63(2): 238-248.

Hamzah, A., Rabu, A., Azmy, R. F. H. R., and Yussoff, N. A. 2010. Isolation and Characterization of Bacteria Degrading Sumandak and South Angsi Oils. Sains Malaysiana. 39(2): 161-168.

Janaki, S., Thenmozhi, S., and Muthumari, R. 2016. A Study on Hydrocarbon Degradation by Biosurfactant Producing Bacillus Cereus in Oil Contaminated Soil Samples. International Journal of Life-Sciences Scientific Research. 2(4): 324-332.

Freitas, B. G., Brito, J. G. M., Brasileiro, P. P. F., Rufino, R. D., Luna, J. M., Santos, V. A., et al. 2016. Formulation of a Commercial Biosurfactant for Application as a Dispersant of Petroleum and by-products Spilled in Oceans. Frontiers in Microbiology. 7(OCT): 1-9.

Sylvester, O., Onyekonwu, M., and Okpokwasili, G. 2019. Isolation and Screening of Hydrocarbon Utilizing Bacteria for Biosurfactant Production: Application for Enhanced oil Recovery. Soc. Pet. Eng. - SPE Niger. Annu. Int. Conf. Exhib. 2019, NAIC 2019.

Wong, S. F., Lim, J. S., and Dol, S. S. 2015. Crude Oil Emulsion: A Review on Formation, Classification and Stability of Water-in-Oil Emulsions. Journal of Petroleum Science and Engineering. 135: 498-504.

Fink, J. 2015 Demulsifiers. Pet. Eng. Guid. to Oil F. Chem. Fluids. 2nd ed., Gulf Professional Publishing. 787-808.

Sabati, H. and Motamedi, H. 2020. Efficient Separation of Water-in-oil Petroleum Emulsions by a Newly Isolated Biodemulsifier Producing Bacterium, Delftia sp. strain HS3. Environmental and Experimental Biology. 18(2): 129-134.

Bafghi, M. K. and Fazaelipoor, M. H. 2012. Application of Rhamnolipid in the Formulation of a Detergent. Journal of Surfactants and Detergents. 15(6): 679-684.

Perfumo, A., Banat, I. M., and Marchant, R. 2018. Going Green and Cold: Biosurfactants from Low-temperature Environments to Biotechnology Applications. Trends in Biotechnology. 36(3): 277-289.

Zarinviarsagh, M., Ebrahimipour, G., and Sadeghi, H. 2017. Lipase and Biosurfactant from Ochrobactrum Intermedium Strain MZV101 Isolated by Washing Powder for Detergent Application. Lipids in Health and Disease. 16(1): 1-13.

Tadros, T. F. 2005. Applied Surfactants: Principles and Applications. Wiley-VCH, Germany.

Cameotra, S. S., Makkar, R. S., Kaur, J., and Mehta, S. K. 2010. Synthesis of Biosurfactants and Their Advantages to Microorganisms and Mankind. R. Sen (Ed.). Biosurfactants Adv. Exp. Med. Biol., Landes Bioscience, Texaspp. 261-280.

Bhat, R., Dayamani, K.J., Hathwar, S., Hedge, R., and Kush, A. 2015. Exploration on Production of Rhamnolipid Biosurfactants Using Native Pseudomonas aeruginosa Strains. Journal of BioScience and Biotechnology. 4(2): 157-166.




How to Cite

Khamis, N., Abdul Rahim, A. ., Ibrahim, N. A. ., & Pahirul Zaman, K. A. K. . (2022). MICROBIAL SURFACTANTS: CLASSIFICATIONS, PROPERTIES, RECOVERY, AND APPLICATIONS. Jurnal Teknologi, 84(2), 35-45. https://doi.org/10.11113/jurnalteknologi.v84.16477



Science and Engineering