• Ika Roostika Tambunan ᵃIndonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, 16111, Indonesia ᵈResearch Center for Horticultural and Estate Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency Jl. Raya-Jakarta Km 46, Cibinong, Bogor 16911, Indonesia
  • Fitri Damayanti Universitas Indraprasta PGRI, Faculty of Math and Science,13760, Jakarta, Indonesia
  • Witjaksono Witjaksono Research Center for Genetic Engineering, Research Organization for Life Sciences and Environment, National Research and Innovation Agency, Jl. Raya-Jakarta Km 46, Cibinong, Bogor 16911, Indonesia



Banana embryogenic callus, reduced temperature, ABA, vitrification, cryopreservation


This paper reports successful preservation methods by minimal growth and cryopreservation of banana embryogenic callus. The minimal growth and cryopreservation techniques can be applied for medium-term and long-term storage. The embryogenic callus of banana cv Dwarf Parfitt was used as the plant material. Different kind of medium formulations, temperature, gelling agents, and ABA concentrations were tested in minimal growth study. The type of explants, dehydration periods, and pre culture media were tested in cryopreservation study. The result showed that the use of WIC medium was better than BM2G for maintaining embryogenic callus. The application of 0.005 mg L-1 ABA combined with reduced temperature (150C) could preserve the cultures longer than 4 months with high survival rate (95%), high recovery rate (90%), and high number of recovered embryos indicating high embryogenic potential. For cryopreservation study, the best dehydration period was 20 minutes. The combined treatments of 150 g/l sucrose and 2 M glycerol at preculture step was the most suitable treatment for successful cryopreservation with 80% survival rates; and the recovered cultures maintained their somatic embryogenic capacity.


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