Textile Effluent Discoloration by Immobilized Phanerochaete Chrysosporium into PVA-Alginate-Sulfate Beads
DOI:
https://doi.org/10.11113/jt.v62.1869Keywords:
PVA-alginate-sulfate beads, Phanerochaete chrysosporium, COD, MnP, SEMAbstract
This paper presents preliminary research on immobilized Phanerochaete chrysosporium in PVA-alginate-sulfate beads to discolor textile effluents. It is an alternative technique from the current physico-chemicals. The main focus of this study was to determine the colour removal, Chemical oxygen deman (COD) removal and manganese peroxidase activity of the immobilized P.crysosporium. Immobilized P.crysosporium also confers advantages such as reusability and improved cell performance. Scanning electron microscope (SEM) was also performed to characterize the immobilization matrix. The immobilized results were compared with that of free cells. Immobilized cells were able to discolor 47.14% compared to free cells which recorded 10.78% colour removal. The COD removal of immobilized cell is more than 60% as compared to that of free cells, which could only reduced 30% of COD. Finally, the manganase peroxidase activities showed a slight difference between the immobilized and free cell at 0.15U/L and 0.13U/L respectively.
References
Meyer, U. 1981. Biodegradation of Synthetic Organic Colorants. Microbial Degradation of Xenobiotics and Recalcitrant Compound, FEMS symposium 12, ed. T. Leisinger, A. M. Cook, R. Hutter & J. Nuesch. London: Academic Press. 371–285.
Zollinger H. 1987. Properties and Application of Organic Dyes and Pigments. Colour Chemistry. New York: VCH Publisher. 92–100.
Deveci, T., Unyayar, A. and Mazmanci, M. A. 2004. Production of Remazol Brilliant Blue R Decolourising Oxygenase from the Culture Filtrate of Funalia Trogii ATCC 200800. Journal of Molecular Catalysis B: Enzymatic. 30(1): 25–32.
Banat, F. A. and Simandl, J. 1996. Removal of Benzene Traces from Contaminated Water by Vacuum Membrane Distillation. Chemical Engineering Science. 51(8): 1257–1265.
Sani, R. K., Azmi, W., Banerjee, U. C. 1998. Comparison of Static and Shake Culture in the Decolorization of Textile Dyes and Dyes Effluents by Phanerochaete Chrysosporium. Folia Microbial. 43(1): 85–88.
Cooper, P. 1993. Removing Color from Dye House Wastewater. Journal of the Society of Dyers and Colourists. 109(3): 97–100.
Stephen, J. A. 1995. Electro-oxidation of Dyestuffs in Waste Water. J.Chem. Technol. Biotechnol. 62(2): 111–117.
Cripps, C., Bumpus, J. A., Aust ,S. D. 1990. Biodegradation of Azo and Heterocyclic Dyes by Phanerochaete Chrysosporium. Appl Environment. Microb. 56(4): 1114–1118.
Ollikka, P., Alhanmaki, K. Leppanen, V-M, Glumoff, T., Raijola, T. Souminen, I. 1993. Decolorization of Azo, Triphenyl Methane, Heterocyclic and Polymeric Dyes by Lignin Peroxidase Isoenzymes from Phanerochaete Chrysosporium. Appl. Environ. Microbial. 59(12): 4010–4016.
Tien, M. and Kirk, T. K. 1983. Lignin-degrading Enzyme from the Hymemocyete Phanerochaete Chrysosporium. Science. 221(4611): 661–663.
Vasdev, K., Kuhad, R. C., Saxena, R. I. 1995. Decolorization of Triphenylmethane Dyes by the Bird’s Nest Fungus Cyathus Bulleri. Current Microbiol. 30(5): 269–272.
Haemmerli, S. D., Leisola, M. S. A., Sanglard, D., Fietcher, A. 1986. Oxidation of Benzo(a) Pyrene by Extracellular Ligninases of Phanerochaete Chrysosporium. J. Biol. Chem. 261(15): 6900–6903.
Cameron, T. P., Hughes, T. I., Kirby, P. E, Fung, V. A., Dunkei, V. C. 1987. Mutagenic Activity of 27 Dyes and Related Chemicals in the Salmonella Microsome and Mouse Lymphoma T K Assay. Mutant. Res. 189(3): 223–261.
Linko, S., Haapala, R. 1996. Progress in Biotechnology. In: Wijffels RH, Buitellar RM, Bucke C, Tramper J, editors. Immobilized cells: Basic and Applications, Amsterdam, The Netherlands: Elsevier. 40-53
Ramakrishnan, S. V., Jamuna, R., Emery, A. N. 1992. Production of Ethanol by Immobilized Yeast Cells. Appl Biochem Biotechnol. 37: 275–282.
Wang Jianlong, Hou Wenhua, Qian Yi. 1995. Immobilization of Microbial Cells Using Polyvinyl Alcohol (PVA)-Polyacrylamide Gels. Biotechnology Techniques. 9(3) :203–208.
Szczesna-Antczak, M. and Galas, E. 2001. Bacillus Subtilis Cells Immobilized in PVA-cryogels. Biomolecular Engineering. 17(2): 55–63.
Idris, A., Mohd Zain, N. A., Suhaimi, M. S. 2008. Immobilization of Baker’s Yeast Invertase in PVA-alginate Matrix Using Innovative Immobilization Technique. Process Biochemistry. 43. 331–338.
Knapp, J. S., Newby, P. S. and Reece, L. P. 1995. Decolorization of Dyes by Wood-Rotting Basidiomycete Fungi. Enzyme and Microbial Technology. 17: 664–668.
Urek, R. O. and Pazarlioglu, N. K. 2007. Enhanced Production of Manganese Peroxidase by Phanerochaete chrysosporium. Brazilian Archives of Biology and Technology. 50: 913–920.
Bergmeyer, H. U. 1974. Methods of Enzymatic Analysis. 2nd ed. New York: Academic Press. 457–458.
Shah, V. and Nerud, F. 2002. Lignin Degradation System of White-Rot Fungi and Its Exploitation for Dye Decolorization. Can. J. Microbiol. 48(10): 857–870.
Wang Bao-e and H. U. Yong-you. 2007. Comparison of Four Support for Adsorption of Reactive Dyes by Immobilized Aspergilus fumigates Beads. Journal of Environmental Sciences. 19(4): 451–457.
Mielgo, I., Moreira, M. T., Feijoo, G., Lema, J. M. 2001. A Packed-Bed Fungal Bioreactor for the Continuous Decolourisation of Azo-dyes (Orange II). Journal of Biotechnology. 89: 99–106.
Rump, H. H. 1999. Laboratory Manual for the Examination of Water, Waste Water and Soil. New York: Wiley. 800.
Hofrichter, M. 2002. Review: Lignin Conversion by Manganase peroxidase (MnP). Enzyme and Microbial Technology. 30(4): 454–466.
Wijetunga Somasiri, Li Xiufen, Wenquan Ruan and Jian Chen. 2008. Colour and COD Removal, Reactor Performance, and Stability in Textile Waste Treatment by Up Flow Anaerobic Sludge Blanket Reactor At Mesophilic Temperature. Electronic Journal of Environment, Agriculture and Food Chemistry. 7(10): 3461–3475.
H’ela, Z., Marc, L., Sami, S. 2002. Degradation of 4-chlorophenol by the White Rot Fungus Phanerochaete chrysosporium in Free and Immobilized Cultures. Bioresour Technol. 84: 145–150.
Glenn, J. K., Akileswaran, L., Gold, M. H. Mn (II) Oxidation is the Principal Function of the Extracellular Mn-peroxidase from Phanerochaete chrysosporium. Archieves of Biochemistry and Biophysics. 198: 251(2): 688–696.
Gold, M. H. and Wariishi, H. 1989. Lignin Peroxidase Compound III Formation, Inactivation, and Conversion to the Native Enzyme. 243(2): 165–168.
Wariishi, H., Vallis, K., and Michael, H. Gold. 1992. Manganese (I1) Oxidation by Manganese Peroxidase from the Basidiomycete Phanerochaete Chrysosporium Kinetic Mechanism and Role of Chelators. The Journal of Biological Chemistry. 267(33): 23688–23695.
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