RECOMBINANT PROTEIN APPLICATION INDUCED DEFENSE MECHANISM IN HOST PLANT AGAINST PAPAYA DIEBACK DISEASE

Ros Azrinawati Hana  Bakar; Norliza  Abu Bakar; Khairulmazmi  Ahmad; Noor Azmi  Shaharuddin; Mohd As’wad  Abdul Wahab; Nor Mustaiqazah  Juri

doi:10.11113/jurnalteknologi.v85.20092

Authors

Ros Azrinawati Hana Bakar ᵃDepartment of Plant Pathology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia ᵇBiotechnology & Nanotechnology Research Centre, Malaysian Agricultural Research & Development Institute, 43400 MARDI Head Quarter Serdang, Selangor, Malaysia https://orcid.org/0000-0003-4291-6757
Norliza Abu Bakar Biotechnology & Nanotechnology Research Centre, Malaysian Agricultural Research & Development Institute, 43400 MARDI Head Quarter Serdang, Selangor, Malaysia
Khairulmazmi Ahmad ᵃDepartment of Plant Pathology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia ᵈInstitute Tropical Agriculture & Food Security (ITAFOS), Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia
Noor Azmi Shaharuddin Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia
Mohd As’wad Abdul Wahab Department of Plant Pathology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia
Nor Mustaiqazah Juri Biotechnology & Nanotechnology Research Centre, Malaysian Agricultural Research & Development Institute, 43400 MARDI Head Quarter Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.11113/jurnalteknologi.v85.20092

Keywords:

Erwinia mallotivora, harpin protein, papaya dieback disease, systemic acquired resistance (SAR)

Abstract

Erwinia mallotivora is a Gram-negative bacterium that causes the papaya dieback disease in Malaysia. Currently, no effective disease control method is documented. In this regard, the adoption of harpin proteins in promoting plant defense mechanisms has been reported to be a promising control method for this disease. This study used, two recombinant harpin proteins, Hrp I and Hrp II, to control the disease in the glasshouse and field conditions. The results of foliar application in the glasshouse showed protective index of 70.8% and 35.7% for Hrp I and Hrp II, respectively. Meanwhile, the field trial for Hrp I showed a promising protection index of 72.3%. Moreover, the plant growth-promoting attributes were also significantly improved in the case of the Hrp I -treated plants. The effectiveness of Hrp I and II as inducers of systemic acquired resistance (SAR) was further validated by profiling defense-related genes using RT-qPCR analysis. The results showed that the treated papaya plants with Hrp I possess the highest expression of defense genes such as peroxidase, osmotin, and PRID in the glasshouse and field samples. In conclusion, the application of Hrp I is a promising disease control approach in managing papaya dieback disease in Malaysia.

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