ANTIDIABETIC PROPERTIES OF DELPHINIDIN 3-SAMBUBIOSIDE-ANTHOCYANIN COMPOUNDS ISOLATED FROM HIBISCUS SABDARIFFA LINN
DOI:
https://doi.org/10.11113/jurnalteknologi.v87.20293Keywords:
Delphinidin 3-sambubioside, Anthocyanin, Compound, Antidiabetic, H. sabdariffa linnAbstract
Delphinidin 3-sambubioside compound (Dp-3-sam) (patent number MY-193036-A) could be effective against diabetes. Results showed that Dp-3-sam caused BRIN BD11, 3T3F442A, L6 myoblast and HSF 1184 cells to become significantly less viable after three days incubation. By comparison to positive control, Dp-3-sam in 50 µg/ml showed significant inhibitory effects 96.76% (p<0.05). Glucose diffusion was significantly diminished by Dp-3-sam in 50 µg/ml concentration (4.98 mmol/L; p<0.01) compared to control (6.85 mmol/L; p<0.05). With regard to wound healing, migration effect exhibited by Dp-3-sam was more pronounced compared to negative control but weaker than positive control during six hours incubation. Meanwhile, 150 µg/mL Dp-3-sam stimulated insulin production 3.15 times more than positive control (p<0.001) at identical two-fold concentration. Moreover, compared to control, Dp-3-sam could not stimulate glucose assimilation in 3T3F442A adipocytes, and neither could enhance GLUT4 diffusion and regulation on L6 myotubes. Results obtained shed new light on anti-diabetes properties of Dp-3-sam in context of herbal medicine.
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