IN SILICO PROTEIN INTERACTION ANALYSIS OF DENGUE VIRUS NON-STRUCTURAL 2A AND HUMAN POTASSIUM CHANNEL KV1.3

Nur Al Syifaa  Hassan; Suzana  Misbah; Siti Aisyah  Razali; Boon-Teong  Teoh; Sazaly  AbuBakar

doi:10.11113/jurnalteknologi.v86.21553

Authors

Nur Al Syifaa Hassan Biological Security and Sustainability (BioSES) Research Interest Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
Suzana Misbah Biological Security and Sustainability (BioSES) Research Interest Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia https://orcid.org/0000-0002-6574-0443
Siti Aisyah Razali Biological Security and Sustainability (BioSES) Research Interest Group, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia https://orcid.org/0000-0002-6180-557X
Boon-Teong Teoh Higher Institution Centre of Excellence (HICoE), Tropical Infectious Diseases Research & Education Centre (TIDREC), Universiti Malaya, 50603 Kuala Lumpur, Malaysia
Sazaly AbuBakar Higher Institution Centre of Excellence (HICoE), Tropical Infectious Diseases Research & Education Centre (TIDREC), Universiti Malaya, 50603 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.11113/jurnalteknologi.v86.21553

Keywords:

Potassium channel, dengue virus, molecular docking, protein-protein interaction, human-virus interaction

Abstract

Dengue virus (DENV) is a mosquito-borne pathogen that causes dengue fever, a potentially severe illness. During infection, DENV interacts with various host factors to facilitate viral production. However, certain host restriction factors, such as the voltage-gated potassium channel Kv1.3, can impede viral replication by limiting DENV entry into host cells. While the interplay of DENV proteins and the specific mechanism facilitating this event remain unclear, our previous yeast two-hybrid interactomes study identified an interaction between DENV non-structural protein 2A (NS2A) and Kv1.3. This study aimed to identify potential binding sites between DENV NS2A and Kv1.3 using in silico approach. Crystal structures of DENV NS2A and Kv1.3 was obtained from RCSB PDB. Protein-protein interaction analysis was conducted using molecular docking with HADDOCK v2.4, and the interaction was assessed based on HADDOCK scores. Our results revealed that the HADDOCK score was -64.7±3.2, indicating an excellent binding affinity between DENV NS2A and Kv1.3. The robust interaction between NS2A and Kv1.3 underscores the need for further investigation into the role of potassium channels in DENV replication.

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