ACTINOBACTERIAL DIVERSITY DETERMINATION IN MALAYSIAN BRIS (BEACH RIDGES INTERSPERSED WITH SWALES) SOIL BY CULTURE-DEPENDENT APPROACH

Authors

  • Hamizah Hazmeen Hairi Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris 35900 Tanjong Malim, Perak, Malaysia
  • Amirah Ahmad Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris 35900 Tanjong Malim, Perak, Malaysia
  • Muhammad Adib Zakwan Azman Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris 35900 Tanjong Malim, Perak, Malaysia
  • Hamidah Idris Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris 35900 Tanjong Malim, Perak, Malaysia
  • Muhd Danish-Daniel Abdullah Institute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia Terengganu 21030 Kuala Nerus, Terengganu, Malaysia
  • Mohd Yazid Hassan Bahagian Pengurusan Sumber Tanah, Jabatan Pertanian Malaysia, Kompleks Pertanian Kuala Terengganu 20050 Kuala Terengganu, Terengganu, Malaysia
  • Noraziah Mohamad Zin Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz 50300 Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.11113/jurnalteknologi.v87.22358

Keywords:

BRIS soil, actinomycetes, actinobacteria, microbial diversity, culture-dependent

Abstract

Globally, the rapid emergence of antibiotic-resistant bacteria poses a major threat to public health, threatening the effectiveness of antibiotics. Addressing the crisis of antimicrobial resistance requires urgency in new drug discovery and the effort in finding novel compounds is dramatically evolving. However, one of the main obstacles is the rediscovery of known compounds from natural resources. To overcome this challenge, exploring underexploited location for new resource of actinomycetes, such as Beach Ridges Interspersed with Swales (BRIS) soil in Malaysia, was selected. Soil samples from Jambu Series and Rudua Series in Setiu, Terengganu were collected and analysed. A total of 87 actinomycetes strains were isolated on yeast malt agar (ISP2), Actinomyces agar and Zobell marine agar, with the highest number from the Jambu series using serial dilution and spread plate method. Antimicrobial screening revealed that 31 isolates exhibited activity against ESKAPE pathogens, with 28 and 12 isolates showing activity against Gram-positive and Gram-negative bacteria, respectively. These isolates showed strong inhibiting response towards one or more ESKAPE pathogens. This study highlights the potential of BRIS soil as an underexploited habitat for discovering actinobacterial communities which could lead to the discovery of a new potential antimicrobial producer to serve as novel drug leads.

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Published

2024-11-11

Issue

Vol. 87 No. 1: Forthcoming Issue January 2025

Section

Science and Engineering

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How to Cite

ACTINOBACTERIAL DIVERSITY DETERMINATION IN MALAYSIAN BRIS (BEACH RIDGES INTERSPERSED WITH SWALES) SOIL BY CULTURE-DEPENDENT APPROACH. (2024). Jurnal Teknologi (Sciences & Engineering), 87(1). https://doi.org/10.11113/jurnalteknologi.v87.22358