POTENTIAL ANTI-AGING AND PHENOLIC COMPOUNDS in DIFFERENT PARTS OF PLEUROTUS PULMONARIUS MUSHROOM EXTRACTS CULTIVATED ON DIFFERENT SUBSTRATES

Authors

  • Nik Nur Azwanida Zakaria ᵃFaculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia ᵇFunctional Agroproduct Research Group, Faculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
  • Norsyafiera Syuhada Fadzil Faculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
  • Zuharlida Tuan Harith ᵃFaculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia ᵇFunctional Agroproduct Research Group, Faculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
  • Kharul Azmi Mua’azam Abdul Rahman Faculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia
  • Mohammad Feizal Daud Field Crops Production and Product Quality Research Group, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA (UiTM) Melaka, Jasin Campus, Melaka, Malaysia
  • Siti Maryam Salamah Ab Rhaman Faculty of Agrobased Industry, Universiti Malaysia Kelantan, 17600 Jeli, Kelantan, Malaysia

DOI:

https://doi.org/10.11113/jurnalteknologi.v87.23064

Keywords:

Pleurotus pulmonarius, bioactivities, phenolics, agriculture sustainability, pineapple waste

Abstract

Pleurotus mushrooms (Pleurotus spp.) are edible fungi known for their rich bioactive compounds and their ability to grow on various lignocellulosic substrates including agriculture byproducts. The unique attributes of Pleurotus spp. have gained popularity, thereby increasing the cultivation of Pleurotus mushroom among farmers. In this study, Pleurotus pulmonarius was cultivated using two different substrates sawdust substrate ((SS) and pineapple-waste substrate (PS)) and separated into caps, stems and stem waste after harvest. The mushrooms parts were extracted using 80% ethanol prior to analysis of the bioactivities and specific phenolic acids.  Anti-aging activities of P. pulmonarius samples were evaluated using enzyme inhibition reactions against tyrosinase, elastase and collagenase. Quantification of phenolic acids was carried out using high-performance liquid chromatography coupled with a diode-array detector (HPLC-DAD). PS-cap samples demonstrated the highest potency against tyrosinase (IC50: 21.22±0.20 mg/mL) and collagenase (IC50: 26.12 ± 1.17 mg/mL), while SS-cap exhibited the highest elastase inhibition atIC50: 36.39 ± 1.93 mg/mL. Additionally, p-coumaric acid was higher in the stem waste parts with 7.37±0.88 mg/g in SS-stem waste and 5.99±0.78 mg/g in PS-stem waste. This study presents the first report on the anti-elastase and anti-collagenase activities of Pleurotus mushroom extract and the potential bioactivities and phenolics in the stem waste extracts. In conclusion, this study highlights the bioactivities and phenolic acid compounds of Pleurotus mushroom extracts cultivated on different substrates, indicating their promising applications in various industries.

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Published

2025-06-13

Issue

Vol. 87 No. 4: July 2025

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Science and Engineering

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POTENTIAL ANTI-AGING AND PHENOLIC COMPOUNDS in DIFFERENT PARTS OF PLEUROTUS PULMONARIUS MUSHROOM EXTRACTS CULTIVATED ON DIFFERENT SUBSTRATES. (2025). Jurnal Teknologi (Sciences & Engineering), 87(4), 765-773. https://doi.org/10.11113/jurnalteknologi.v87.23064