INTEGRATED HIGH MAGNETIC GRADIENT SYSTEM FOR TRAPPING NANOPARTICLES
DOI:
https://doi.org/10.11113/jt.v75.5344Keywords:
V-shaped magnetic core, Lab-on-chip (LoC), high magnetic gradient, bioparticles, microfluidics, nanoparticlesAbstract
Lab-on-chip (LoC) magnetic separator is important in clinical diagnostics and biological studies where different types of biological cells need to be isolated from its heterogeneous population. In this work, a novel design of on-chip V-shaped magnetic core generating high magnetic gradient and force for trapping magnetically labelled bioparticles is presented. The integrated magnetic system consisted of a spiral-shaped planar microcoils and a V-shaped permalloy (Ni80Fe20) magnetic core structure, which was designed to be part of LoC separator system. The effects of V-shaped magnetic core tip area, the current injection to the microcoils on the magnetic field, as well as its gradient and force on magnetic nanoparticles were simulated and analyzed. Finite element analysis (FEA) simulation using two dimensional (2D) axial symmetry model and steady state analysis of the DC magnetostatics module confirmed the effect of V-shaped magnetic core tip on the high magnetic field generation. The smallest V-shaped magnetic core tip area and the highest current injected to the magnetic coils had significantly amplified the magnetic flux density, its gradient, and the magnetic force generated on a magnetic nanoparticles. Functional test results justified the proportional relationship between DC applied and the trapping area of the magnetic nanoparticles. Effective separation of biological cells tagged with magnetic nanoparticles in LoC system was expected with integration of this high gradient on-chip magnetic system.
References
Lim, Y. C., Kouzani, A. Z. and Duan, W. 2010. Lab-on-a-chip: a Component View. Microsystem Technologies. 16 (12): 1995–2015
Ganguly, R. and Puri, I. K. 2010. Microfluidic Transport in Magnetic MEMS and BioMEMS. Wiley Interdisciplinary Reviews. Nanomedicine Nanobiotechnology. 2(4): 382–99
Pankhurst, Q. A., Connolly, J., Jones, S. K. and Dobson, J. 2003. Applications of Magnetic Nanoparticles in Biomedicine. Journal of Physics. D. Applied Physics. 36 (13): R167–R181
Cao, Q., Han, X. and Li, L. 2014. Configurations and Control of Magnetic Fields for Manipulating Magnetic Particles in Microfluidic Applications: Magnet Systems and Manipulation Mechanisms. Lab Chip. 14(15): 2762–77
Ramadan, Q., Samper, V., Poenar, D. P. and Yu, C. 2006. An Integrated Microfluidic Platform for Magnetic Microbeads Separation and Confinement. Biosensors Bioelectronics. 21 (9): 1693–702
Ramadan, Q., Samper, V. D., Puiu, D. P. and Yu, C. 2006. Fabrication of Three-Dimensional Magnetic Microdevices With Embedded Microcoils for Magnetic Potential Concentration. Journal Microelectromechanical Systems. 15 (3): 624–638
Smistrup, K., Tang, P. T., Hansen, O. and Hansen, M. F. 2006. Microelectromagnet for Magnetic Manipulation in Lab-on-a-chip Systems. Journal of Magnetism and Magnetic Materials. 300 (2): 418–426
Inglis, D. W., Riehn, R., Sturm, J. C. and Austin, R. H. 2006. Microfluidic High Gradient Magnetic Cell Separation. Journal of Applied Physics. 99 (8): 08K101
Kong, H. S. E, Sugiarto, H. S., Liew, H. F., Wang, X., Lew, W. S., Nguyen, N.-T. and Chen, Y. 2010. An Efficient Microfluidic Sorter: Implementation of Double Meandering Micro Striplines for Magnetic Particles Switching. Microfluid Nanofluidics. 10 (5): 1069–1078
Fulcrand, R., Jugieu, D., Escriba, C., Bancaud, A., Bourrier, D., Boukabache, A. and Gué, A.M. 2009. Development of a Flexible Microfluidic System Integrating Magnetic Micro-actuators for Trapping Biological Species. Journal of Micromechanics Microengineering. 19(10): 105019
Dong, T., Su, Q., Yang, Z., Karlsen, F., Jakobsen, H., Egeland, E. B. and Hjelseth, S. 2010. Fully Integrated Micro-Separator with Soft-Magnetic Micro-pillar Arrays for Filtrating Lymphocytes. Conference Proceeding of IEEE Engineering Medical Biology Society. Buenos Aires, Argentina. 6522–6526
Ramadan, Q., Poenar, D. P. and Yu, C. 2008. Customized Trapping of Magnetic Particles. Microfluid. Nanofluidics. 6 (1): 53–62
Xia, N. , Hunt, T. P., Mayers, B. T., Alsberg, E., Whitesides, G. M., Westervelt, R. M. and Ingber, D. E. 2006. Combined Microfluidic-micromagnetic Separation of Living Cells in Continuous Flow. Biomedical Microdevices. 8(4): 299–308
Yapici, M. K. and Zou, J. 2008. Permalloy-coated Tungsten Probe for Magnetic Manipulation of Micro Droplets. Microsystem Technologies. 14(6): 881–891
Barbic, M., Mock, J. J., Gray, A. P. and Schultz, S. 2001. Scanning Probe Electromagnetic Tweezers. Applied Physics Letters. 79. (12): 1897
Matthews, B. D., LaVan, D. a., Overby, D. R., Karavitis, J. and Ingber, D. E. 2004. Electromagnetic Needles with Submicron Pole Tip Radii for Nanomanipulation of Biomolecules and Living Cells. Applied Physics Letters. 85(14): 2968
Pamme, N., Eijkel, J. C. T. and Manz, A. 2006. On-chip Free-flow Magnetophoresis: Separation and Detection of Mixtures of Magnetic Particles in Continuous Flow. Journal of Magnetism Magnetic Materials. 307(2): 237–244
Pamme, N. 2006. Magnetism and Microfluidics. Lab Chip. 6 (1): 24–38
Shafique M. and Hansen, M. F. 2003. Modeling the Magnetic Field From a Microelectromagnet in FEMLAB. Proceedings from the Nordic MATLAB Conference. Copenhagen, Denmark. 209–214
COMSOL. Introduction to ACDC Module. 2014
de Vries, A. H. B., Krenn, B. E., van Driel, R. and Kanger, J. S. 2005. Micro Magnetic Tweezers for Nanomanipulation Inside Live Cells. Biophysics Journal. 88(3): 2137–44
Fulcrand, R., Bancaud, A., Escriba, C., He, Q., Charlot, S., Boukabache, A. and Gué, A.-M. 2011. On chip Magnetic Actuator for Batch-mode Dynamic Manipulation of Magnetic Particles in Compact Lab-on-chip. Sensors Actuators B Chemicals. 160(1):1520–1528,
Teste, B., Malloggi, F., Gassner, A.-L., Georgelin, T., Siaugue, J.-M., Varenne, A., Girault, H. and Descroix, S. 2011. Magnetic Core Shell Nanoparticles Trapping in a Microdevice Generating High Magnetic Gradient. Lab Chip. 11(5): 833–4
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