PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum

Authors

  • Suleiman Danladi School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
  • Amirah Wan-Azemin School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
  • Yahaya Najib Sani School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
  • Khamsah Suryati Mohd Agriculture Production and Food Innovation Research Institute (AGROPOLIS), Universiti Sultan Zainal Abidin, Tembila Campus, 22200 Besut, Terengganu, Malaysia
  • Mahadeva Rao US School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia
  • Sharif Mahsufi Mansor Centre of Drug Research, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia
  • Saravanan Dharmaraj School of Basic Medical Sciences, Faculty of Medicine, Universiti Sultan Zainal Abidin, Medical Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Terengganu, Malaysia

DOI:

https://doi.org/10.11113/jt.v77.5988

Keywords:

Melastoma malabathricum, DPPH, phenolics, flavonoid, quercetin

Abstract

Melastoma malabathricum Linn. commonly known as ‘senduduk’ in Malaysia, belongs to Melastomataceae family. The study was carried out to screen the phytochemicals present in methanol extract of leaf, flower, fruit, and stem and to evaluate the antioxidant activity, total flavonoid and total phenolic contents of these different parts. Phytochemical screening showed that all parts of this plant contained tannins, steroids, phenols and flavonoids. The flower extract was found to have highest total phenolic whereas the leaf demonstrated the highest flavonoid content followed by flower. The results of antioxidant activity from the DPPH assay showed that the flower has the highest radical scavenging activity comparable to quercetin standard while stem showed the lowest activity. The higher content of total phenolics, flavonoids and antioxidant capacity of flower suggests the possibility of its incorporation and exclusion of stem in M. malabathricum preparations for development of newer effective drugs.

References

Ong, H. C. and Norzalina J. 1999. Malay Herbal Medicine in Gemencheh, Negri Sembilan, Malaysia. Fitoterapia. 70(1): 10-14.

Zakaria, Z. A., Rofiee, M. S., Mohamed, A. M., Teh, L. K. and Salleh, M. Z. 2011. In Vitro Antiproliferative and Antioxidant Activities and Total Phenolic Contents of the Extracts of Melastoma Malabathricum Leaves. Journal of Acupuncture and Meridian Studies. 4(4): 248-56.

Triantaphyllou, K., Blekas, G. and Boskou, D. 2001. Antioxidative Properties of Water Extracts Obtained from Herbs of the Species Lamiaceae. International Journal of Food Sciences and Nutrition. 52(4): 313-317.

Pietta, P. G. 2000. Flavonoids as Antioxidants. Journal of Natural Products. 63(7): 1035-1042.

Heim, K. E., Tagliaferro, A. R. and Bobilya, D. J. 2002. Flavonoid Antioxidants: Chemistry, Metabolism and Structure-Activity Relationships. Journal of Nutritional Biochemistry. 13(10): 572-584.

Halliwell, B., Murcia, M. A., Chirico, S. and Aruoma, O. I. 1995. Free Radicals and Antioxidants in Food and In Vivo: What They Do and How They Work. Critical Reviews in Food Science and Nutrition. 35(1-2): 7-20.

Ooi, V. E. C. and Liu, F. 1999. A Review of Pharmacological Activities of Mushroom Polysaccharides. International Journal of Medicinal Mushrooms. 1(3): 195-206.

Halliwell, B., Aeschbach, R., Löliger, J. and Aruoma O. I. 1995. The Characterization of Antioxidants. Food and Chemical Toxicology. 33(7): 601-617.

Riaz, T., Abbasi, A., Aziz-Ur-Rehman, Shahzadi, T., Ajaib M. and Khan, K. M. 2012. Phytochemical Screening, Free Radical Scavenging, Antioxidant Activity and Phenolic Content of Dodonaea Viscosa. Journal of the Serbian Chemical Society. 77(4): 423-435.

Susanti, D., Sirat, H. M., Ahmad, F., Ali, R. M., Aimi, N. and Kitajima, M. 2007. Antioxidant and Cytotoxic Flavonoids from the Flowers of Melastoma Malabathricum L. Food Chemistry. 103(3): 710-716.

11. Nishanthini, A., Balamurugan, K. and Mohan, V. R. 2013. Evaluation of Hepatoprotective and Antioxidant Activity of Melastoma. International Journal of Current Pharmaceutical Research. 5(1):33–41.

Joffry, S. M., Yob, N. J., Rofiee, M. S., Affandi, M. M. R. M. M., Suhaili, Z. and Othman F. 2012. Melastoma Malabathricum (L.) Smith Ethnomedicinal Uses, Chemical Constituents, and Pharmacological Properties: A Review. Evidence-based Complementary and Alternative Medicine. 2012: 1-48. doi: 10.1155/2012/258434.

Indubala, J. and Ng, L. T. 2000. Herbs: The Green Pharmacy of Malaysia. Kuala Lumpur: Vinpress Sdn. Bhd , 76-77.

Kokate A. 1999. Phytochemical Methods. Phytotherapy. 78: 126-129.

Evans, W. C. 1997. An index of medicinal plants. In: A Textbook of Pharmacognosy. 14th ed. London: Bailliere Tindall Ltd.

Wagner, H. 1993. Pharmazeutische Biology. 5th ed. Stuttgart: Gustav Fisher Vwelag.

Richardson, P. M. and Harborne, J. B. 1990. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd ed. London: Chapman and Hall.

Schalbert, A., Monties, B. and Janin, G. 1989. Tannins in Wood: Comparison of Different Estimation Methods. Journal of Agricultural and Food Chemistry. 37: 1324-1329.

Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the Free Radical-Scavenging Activity of Propolis From Several Regions of Argentina. Journal of Ethnopharmacology. 71: 109-114.

Miser-Slihoglu, E., Akaydin, G., Caliskan-Can, E. and Yardim-Akaydin, S. 2013 Evalution of Antioxidant Activity of Various Herbal Folk Medicines. Journal of Nutrition and Food Science. 3(5): 222.

Susanti, D., Sirat, H. M., Ahmad, F. and Ali R. M. 2008. Bioactive Constituents From the Leaves of Melastoma. Jurnal Ilmiah Farmasi. 5(1): 1-8.

Cavin, A., Dyatmyko, W. and Hostettmann, K. 1999. Screening of Indoesian Plants for Antifungal and Free Radical Scavenging Activities. Pharmaceutical Biology. 37(4): 260-268.

Mamat, S. S., Kamarolzaman, M. F. F., Yahya, F., Mahmood, N. D., Shahril, M. S. and Jakius, K. F. 2013. Methanol Extract of Melastoma Malabathricum Leaves Exerted Antioxidant and Liver Protective Activity in Rats. BMC Complementary and Alternative Medicine.13: 326.

Karupiah, S. and Ismail, Z. 2013. Antioxidative Effect of Melastoma Malabathticum L Extract and Determination of its Bioactive Flavonoids from Various Location in Malaysia by RP-HPLC with Diode Array Detection. Journal of Applied Pharmaceutical Science. 3(2): 19-24.

Fernando, I. D. N. S., Abeysinghe, D. C., and Dharmadasa, R. M. 2013. Determination of Phenolic Contents and Antioxidant Capacity of Different Parts of Withania Somnifera (L.) Dunal. From Three Different Growth Stages. Industrial Crops and Products. 50: 537-539.

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Published

2015-10-28

How to Cite

PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum. (2015). Jurnal Teknologi, 77(2). https://doi.org/10.11113/jt.v77.5988