OPTIMIZATION OF SOXHLET EXTRACTION PARAMETER OF Annona muricata LEAVES USING BOX-BEHNKEN DESIGN (BBD) EXPERT AND ANTIOXIDANT ANALYSIS
DOI:
https://doi.org/10.11113/jt.v77.6001Keywords:
Annona muricata, Soxhlet extraction, HPLC, Box-Behnken designAbstract
Response surface methodology (RSM) was employed to optimize the extraction of herbal plant of Annona muricata L. using Box-Behnken Design (BBD). The Box-Behnken Design (BBD) with three factors and two responses (yield and total solid content) was implemented. The processing parameters of A. muricata leaves by soxhlet extraction were solvent to raw material ratio, ethanol concentration, and duration of extraction using soxhlet extractor. The presence of acetogenins compounds was screened by High Performance Liquid Chromatography. Optimum condition with the solvent to raw material ratio (1:5.92), ethanol concentration (1.67 %), and duration of extraction (6.84 hours) was obtained and further analyzed with antioxidant tests. Analyses showed the A. muricata leaves contained high total phenolic and flavonoid contents which corresponding to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test. The DPPH result showed that the extract has the highest percentage of scavenging activity with the inhibitory concentration (IC50) value of leaves is 0.243 mg/mL + 0.001 nearly comparable to butylated hydroxyanisole (BHA) and ascorbic acid (Vit. C), which indicated that the leaves also have higher free radical scavenging activity compared to these commercial standards. Besides, the presence of primary; polysacharide (21.85 % + 0.001), protein (19.59 % + 0.000), glycosaponin (18.87 % + 0.001) and secondary; phenolic (47.26 mg GAE/1g + 0.001), flavonoid (22.12 mg CE/1g + 0.001) metabolites in A. muricata leaves suggested that the leaves contained strong antioxidant properties that are believed can act as anticancer agent for the body.
References
Philips, K., Sri Nurestri, A. M., Sani, W., Shin, S. K., Kumar, S., Lai, H. S., Serm, L. G., and Syarifah, N. S. A. R. 2009. Antimicrobial Activity of Some Medicinal Plant from Malaysia. American Journal of Applied Sciences. 6(8): 1613-1617.
Adjanohoun, J. E., Aboubakar, N., Dramane, K., Ebot, M. E., Ekpere, J. A., Enow-Orock, E. G., Focho, D., Gbile, Z. O., Kamanyi, A., Kamsu Kom, J., Keita, A., Mbenkum, I., Mbi, C. N., Mbiele, A. L., Mbome, I. L., Mubiru, N. K., Nancy, L., Nkongmeneck, B., Satabie, B., Sofowora, A., Tamze, N., and Wirmum, C. K. 1996. Traditional Medicine and Pharmacopoeia: Contribution to Ethnobotanical and Floristic Studies in Cameroon. OAU/STRC. 641.
Taylor, L. 2002. Graviola. Technical Data Report for Graviola, Annona Muricata. Sage Press, Austin. 1-18.
Lans, C. A. 2006. Ethnomedicines Used in Trinidad and Tobago for Urinary Problems and Diabetes Mellitus, Journal of Ethnobiology and Ethnomedicine. 2(52): 45-55.
Berlowski, A., Zawada, K., Wawer, I. and Paradowska, K. 2013. Antioxidant Properties of Medicinal Plants from Peru. Food and Nutrition Sciences. 4: 71-77.
Ruddon, R. W. 2007. Cancer. Cancer Biology. 4th Edition. Oxford University Press, Oxford. 1-6.
Rachmani, E. P. N., Suhesti, T. S., Widiastuti, R. and Aditiyono. 2012. The Breast of Anticancer from Leaf Extract of Annona Muricata Against Cell Line in T47D. International Journal of Applied Science and Technology. 2(1): 157-164.
Suyatmi, Suselo, Y. H. and Jusuf, S. A. 2012. The Selective Cytotoxicity of Ethanolic Extract of Annona muricata Leaf on HeLa Cervical Cancer Cells. International Conference: Research and Application on Traditional Complementary and Alternative Medicine in Health Care (TCAM). 24-27.
Adewole, S. O., Caxton-Martins, E. A. and Ojewole, J. A. O. 2006. Histochemical and Biochemical Effects of Melatonin on Pancreatic β-cells on Streptozocin Treated Diabetic Rats. Pharmacology-Online. 2: 1-21.
Yang, H., Li, X., Tang, Y., Zhang, N., Chen, J. and Cai, B. 2009. Supercritical Fluid CO2 Extraction and Simultaneous Determination of Eight Annonaceous Acetogenins in Annona Genus Plant Seeds by HPLC-DAD Method. Journal of Pharmaceutical and Biomedical Analysis. 49: 140-144.
Olayinka, O. O., Kareem, A. M., Ariyo, I. B., Omotugba, S. K. and Oyebanji, A. O. 2012. Antioxidant Contents (Vitamin C) of Raw and Blenched Different Fresh Vegetable Samples. Food and Nutrition Sciences. 3: 18-21.
Weisburger, J. H. 1999. Carcinogenicity and Mutagenicity Testing, Then and Now. Mutation Research. 437: 105-112.
Shao, P., Sun, P. and Ying, Y. 2008. Response Surface Optimization of Wheat Germ Oil Yield by Supercritical Carbon Dioxide Extraction. Journal of Food Bioproduct Process. 86: 227-231.
Pericin, D., Radulovic-Popovic, L. J., Vastag, Z., Madarev-Popovic, S. and Trivic, S. 2009. Enzymatic Hydrolysis of Protein From Hull-Less Pumpkin Oil Cake: Application of Response Surface Methodology. Journal of Food Chemistry. 115: 753-757.
Thaipong, K., Boonprakob, U., Crosby, K., Zevallos, L. C. and Byrne, D. H. 2006. Comparison of ABTS, DPPH, FRAP and ORAC Assays for Estimating Antioxidant Activity from Guava Fruit Extracts. Journal of Food Composition and Analysis. 19: 669-675.
Cantin, C. M., Moreno, M. A. and Gogorcena, Y. 2009. Evaluation of the Antioxidant Capacity, Phenolic Compounds, and Vitamin C Content of Different Peach and Nectarine [Prunus Persica (L.) Batsch] Breeding Progenies. Journal of Agricultural and Food Chemistry. 57: 4586-4592.
Norshazila, S., Syed Zahir, I., Mustapha Suleiman, K., Aisyah, MR. and Kamarul Rahim, K. 2010. Antioxidant Levels and Activities of Selected Seeds of Malaysian Tropical Fruits. Malaysian Journal of Nutrition.16(1): 149-159.
Chen, Y., Roan, H., Lii, C., Huang, Y. and Wang, T. 2011. Relationship between Antioxidant and Antiglycation Ability of Saponins, Polyphenols and Polysaccharides in Chinese Herbal Medicines used to Treat Diabetes. Journal of Medicinal Plants Research. 5 (11): 2322-2331.
Dulekgurgen, E. 2004. Proteins (Lowry) Protocol. Proteins Protocol. UIUC. 1-5
Malaysian Standard. 2010. MS No. 07U001R0, Phytopharmaceutical Aspect of Freeze Dried Water Extract from Tongkat Ali Roots-Specification. Department of Standards Malaysia. 1-13.
Goel, N., Siroshi, S. K. and Dwivedi, J. 2012. Estimation of Total Saponins and Evaluate Their Effect on in Vitro Methanogenesis and Rumen Fermentation Pattern in Wheat Straw Based Diet. Journal of Advanced Veterinary Research. 2: 120-126.
Yunus, M. A. C., Yaw, C. L. and Idham, Z. 2011. Effects of Variables on the Production of Red-fleshed Pitaya Powder using Response Surface Methodology. Jurnal Teknologi. 56: 15-29.
Machatha, S. G. 2006. Estimation of Ethanol/Water Solubility Profiles (Doctoral dissertation). The University of Arizona. Tucson. Arizona. United States.
Yang, Z., Luo, S., Peng Q., Zhao, C., Yu, Z. 2007.GC-MS Analysis of the Essential Oil of Coral Ginger (Zingiber corallinum Hance) Rhizome Obtained by Supercritical Fluid Extraction and Steam Distillation Extraction. Gromatographia. 69: 785-790.
Geankoplis, C. J. 2003. Transport Process and Separation Process Principle. 4th ed. New Jersey:pearson Education, Inc
Amin, I., Tiong, N., Tan, S., and Azrina, A. 2009. Antioxidant Properties of Selected Non-Leafy Vegetables. NFS. 39(2): 176-180.
Bobadilla, M., Zavala, F., Sisniegas, M., Zavaleta, G., Mostacero, J., Taramona, L. 2005 Evaluación larvicida de suspensiones acuosas de Annona muricata Linnaeus (guanábana) sobre Aedes aegypti Linnaeus (Diptera, Culicidae). Revista Peruana de Biologia. 12: 15-152.
Singh, D. 2014. Advances in Plant Biopesticides. India. Springer.
Shabir, G., A. 2010. A New Validated HPLC Method for the Simultaneous Determination of 2-phenoxyethanol, Methylparaben, Ethylparaben and Propylparaben in a Pharmaceutical Gel. Indian Journal of Pharmaceutical Sciences. 72(4): 421-425.
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