THE ROLE OF POLYAMINES IN ANTI-PROLIFERATIVE EFFECT OF SELECTED MALAYSIAN HERBS IN HUMAN LUNG ADENOCARCINOMA CELL LINE
DOI:
https://doi.org/10.11113/jt.v77.6753Keywords:
Cancer, A549, polyamines, natural product, growth inhibitionAbstract
Polyamine is a group of aliphatic amine that is necessary for cell growth and development. However, potential of developing cancer is enhanced with the increase of polyamine levels in the body. Since polyamines can be obtained through diet intake, it is essential for cancer patients to consume foods that have low level of polyamines. Therefore, this study aims to determine the polyamine level of selected plants; Sabah Snake Grass (Clinacanthus nutans), Daun Kadok (Piper sarmentosum) and Fig (Ficus auriculata). The antiproliferative effects of C. nutans, P. sarmentosum and F. auriculata were investigated using MTT assay and the polyamines content in these plants and intracellular content were quantified using High Performance Liquid Chromatography (HPLC). The results showed that polyamines content in C. nutans (41.49 nmol/g), P. sarmentosum (55.01 nmol/g) anf F. auriculata (39.28 nmol/g) were classified under low level. The IC50 values for these plants were in the range of 20-30 mg/ml. This study demonstrated that these plants inhibit the A549 cell’s growth after 24 hours to 96 hours of exposure. Depletion of intracellular polyamine after 48 hours to 96 hours of exposure was also identified. In conclusion, the study showed that C. nutans, P. sarmentosum and F.auriculata contain low level of polyamine and have anti-proliferative effect against A549 cells. Therefore these plants are recommended to reduce or delayed the occurrence of malignancy and prevention of cancer recurrence after successful treatment.
References
Siegel, R., Naishadham, D., & Jemal, A. 2013. Cancer Statistics. A Cancer Journal for Clinicians. 63(1): 11-30.
Palmer, A. J, Ghani, R. A, Phanstiel, O., Wallace, H. M. 2009. A Putrescine-Anthracene Conjugate: A Paradigm for Selective Drug Delivery. Biochem J. 424: 431-438.
Coffino, P. & Poznanski, A. 1991. Killer Polyamines. Journal of Cellular Biochemistry. 45: 54-58.
Minois, N., Carmona-Gutierrez, D., & Madeo, F. 2011. Polyamines in Aging and Disease. Aging. 3(8): 716-32.
Nowotarski, S. L., Woster, P. M., & Jr, R. A. C. 2013. Polyamines and Cancer : Implications for Chemotherapy And Chemoprevention. Expert Reviews in Molecular Medicine. 15: 1-21.
Steward, W. P., & Brown, K. 2013. Cancer Chemoprevention: A Rapidly Evolving Field. British Journal of Cancer. 109(1): 1-7. doi:10.1038/bjc.2013.280.
Abdurrahman, A. D., Qureshi, S., Mohammad, F.Q, Viquar, F.Q. & Mohammad, R.Q. 2012. Experimental Approach to Treatment of Colorectal Cancer by Herbs and Their Constituents: An Overview on in vivo and in vitro Protocols and Molecular Targets. International Journal of Cancer Research. 37-48.
Rathnasamy, S., Kamaruzaman, M., Shaida, F. S., & Akinboro, A. 2013. Evaluation of Cytotoxic , Mutagenic and Antimutagenic Potential of Leaf Extracts of Three Medicinal Plants Using Allium cepa chromosome assay. International Current Pharmaceutical Journal. 2(8): 131-140.
Yin, M. 2013. Development of Natural Antitumor Agents. BioMedicine. 3(3): 105.
Cipolla, B. G., Havouis, R., & Moulinoux, J. P. 2007. Polyamine Contents in Current Foods: A Basis for Polyamine Reduced Diet and a Study of Its Long Term Observance and Tolerance in Prostate Carcinoma Patients. Amino Acids. 33: 203-212.
Agostinelli, E., Marques, M. P. M., Calheiros, R., Gil, F. P. S. C., Tempera, G., Viceconte, N., Battaglia, V., Grancara, S., & Toninello, A. 2010. Polyamines: Fundamental Characters in Chemistry and Biology. Amino Acids. 38: 393-403.
D’Adamo, P. J. 2002. The Eat Right 4 Your Type. 495-554.
Bardocz, S. 1995. Polyamines in Food And Their Consequences For Food Quality And Human Health. Trends Food Science Technology. 6: 341-346.
Soda, K., Kano, Y., Sakuragi, M., Takao, K., Lefor, A., & Konishi, F. 2009. Long-term Oral Intake Increases Blood Polyamine Concentrations. J Nutr Sci Vitaminol (Tokyo). 55: 361-366.
Soda, K., Kano, K., & Chiba, F. 2012. Food Polyamine and Cardiovascular Disease: An Epidemiology Study. Global Journal of Health Science. 4(6): 170-178.
Agostinelli, E., Marques, M. P. M., Calheiros, R., Gil, F. P. S. C., Tempera, G., Viceconte, N., Battaglia, V., Grancara, S., & Toninello, A. 2010. Polyamines: Fundamental Characters in Chemistry and Biology. Amino Acids. 38: 393-403.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
