A STUDY OF CROSS-CONTAMINATION OF FOODBORNE PATHOGENS ON THE KITCHEN SURFACES

Authors

  • Murni Noor Al Amin Biofilm Research, Biotechnology Research Alliances, Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Wan Rosmiza Zana Wan Dagang Biofilm Research, Biotechnology Research Alliances, Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

DOI:

https://doi.org/10.11113/jt.v77.6883

Keywords:

Cross-contamination, foodborne pathogens, kitchen surfaces

Abstract

Cross-contamination provides the opportunity for various of bacteria to be deposited on each of the surface contact during meal preparation. Raw poultry especially raw chicken was the main reservoir of foodborne pathogens that can cause foodborne diseases. Therefore, a study on the potential of cross-contamination contribute to spread E. coli, Salmonella spp. and S. aureus on the kitchen surfaces during chicken preparation was conducted. A total of 36 isolates were collected from six sampling sites before and after the chicken preparation. The enumeration of the bacteria from the sampling sites showed a significant change in the mean total plate counts (TPC) of the isolates before and after the chicken preparation. These results emphasized that cross-contamination occurred around the sampling sites during the preparation of the chicken. Isolation and identification of the three foodborne pathogens, E. coli, Salmonella spp. and S. aureus were carried out on its respectively selective and differential media. The presumptive identified foodborne pathogens were confirmed as E. coli, Salmonella spp. and S. aureus according to their microscopic and biochemical characteristics.

References

C. W. Hedberg, W. C. Levine, K. E. White, R. H. Carlson, D. K. Winsor, D. N. Cameron, K. L. McDonald, M. T. Osterholm. 1992. JAMA. 268: 3208.

H. Kusumaningrum, G. Riboldi, W. Hazeleger, R. Beumer. 2003. Int. J. Food Microbiol. 85: 227.

G. Moore, I. S. Blair, D. A. McDowell. 2007. J. Food Prot. 70: 2273.

P. Teixera, S. Silva, F. J. Araújo, Azeredo and R. Oliveira. 2007. Bacterial Adhesion to Food Contacting Surfaces. A. Méndez-Vilas (Ed.). Communicating Current Research and Educational Topics and Trends inApplied Microbiology. FORMATEX Research Centre.

J. C. De Wit, G. Brockhuizen, E. H. Kampelmacher. 1979. J. Hyg. 83: 27.

E. Scott, S. F. Bloomfield. 1990. J. Appl. Bacteriol. 68: 271.

R. Gorman, S. Bloomfield, C. C. Adley. 2001. J. Food Microbiol. 76: 143.

P. Rusin, S. Maxwell, C. Gerba. 2002. J. Appl Microbiol. 95: 585.

J. V. S. Pether, R. J. Gilbert, R. J. 1971. J.Hyg. 69: 673.

X. P. Jiang, M. P. Doyle. 1999. J. Food Prot. 62: 805.

H. D. Kusumaningrum, M. M. van Putten, F. M. Rombouts, R. R. Beumer. 2002. J. Food Prot. 65: 61.

L. G. Brown, S. Khargonekar, L. Bushnell, The Environmental HealthSpecialist Network Working Group. 2013. J. Food Prot. 76: 2141.

T. Mondal, M. S. R. Khan, M. Alam, M. Purakayastha, M. Das, M. P.Siddique. 2008. Bangladesh J. Vet. Med. 6: 7.

M. Zahera, C. Rastogi, P. Sigh, S. Iram, S. Khalid, A. Kushwaha. 2011. Eur. J. Exp. Biol. 1: 118.

J. C. Holt, N. R. Kreig, P. H. A. Sneath, J. Y. Stanley. 1994. Bergey's Manual of Determinative Bacteriology. USA: Springer.

S. T. Odonkor, J. K. Ampofo. 2013. J. Microbiol. Res. 4: 5.

C. Bell, P. Neaves, A. P. Williams. 2005. Food Microbiology and Laboratory Practice. United Kingdom: Blackwell Publishing.

A. Hwang, L. Huang, L. 2010. Ready-To-Eat Foods: Microbial Concerns and Control Measures. United State of America: CRC Press: Taylor and Francis Group.

M. H. Mirmomeni, S. Naderi, A. H. Colagar, S. Sisakhtnezhad. 2009. Res. J. Biol. Sci. 4: 656.

P. Singh, A. Prakash. 2008. ActaagriculturaeSlovenica. 92: 83.

M. J. Leboffe, B. E. Pierce. 2010. Microbiology: Laboratory Theory and Application. Englewood: Morton Publishing Company.

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Published

2015-12-20

Issue

Section

Science and Engineering

How to Cite

A STUDY OF CROSS-CONTAMINATION OF FOODBORNE PATHOGENS ON THE KITCHEN SURFACES. (2015). Jurnal Teknologi, 77(31). https://doi.org/10.11113/jt.v77.6883