THE EFFECT OF DIFFERENT PHOSPHATE CONCENTRATION ON GROWTH, LIPID PRODUCTIVITY AND METHYL PALMITATE METHYL ESTER PRODUCTION BY NANNOCHLOROPSIS OCULATA
DOI:
https://doi.org/10.11113/jt.v77.6915Keywords:
Biodiesel, Microalgae, Nannochloropsis oculata, phosphateAbstract
This study reports the effect of different phosphate concentration ranging from 0 g/L, 0.1 g/L, 0.2 g/L, 0.5 g/L, 1.0 g/L and 2.0 g/L on the growth and lipid productivity of the microalgae Nannochloropsis oculata. Based on the result, it shows that, the microalgae growth increased as the concentration of phosphate added into the medium increased while the percentage of lipid produced was inversely proportional to increasing concentrations of phosphate. The highest amount of lipid produced was when the microalgae were cultured under 0.1 g/L phosphate which was 5.7%. Based on the standard analyzed using gas chromatography, the percentage of methyl palmitate methyl ester produced increased along with increasing concentration of phosphate until 0.5 g/L which was 11%, however further increase in phosphate concentration caused the lipid productivity to drop.
References
Y. Chisti. 2007. Biodiesel From Microalgae. Biotechnology Advances. 25: 294-306.
B. X. Peng, Q. Shu, J. F. Wang, G. R. Wang, D. Z. WangM. H. Han. 2008. Biodiesel Production From Waste Oil Feedstocks By Solid Acid Catalysis. Process Safety and Environmental Protection. 86: 441-447.
C. C. EnweremaduM. M. Mbarawa. 2009. Technical Aspects Of Production And Analysis Of Biodiesel From Used Cooking Oil—A Review. Renewable and Sustainable Energy Reviews. 13: 2205-2224.
T. T. Y. Doan, B. SivaloganathanJ. P. Obbard. 2011. Screening Of Marine Microalgae For Biodiesel Feedstock. Biomass and Bioenergy. 35: 2534-2544.
H. M. Amaro, A. C. GuedesF. X. Malcata. 2011. Advances And Perspectives In Using Microalgae To Produce Biodiesel. Applied Energy. 88: 3402-3410.
S. Renaud, D. Parry, L. V. Thinh, C. Kuo, A. PadovanN. Sammy. 1991. Effect Of Light Intensity On The Proximate Biochemical And Fatty Acid Composition Of Isochrysis Sp. And Nannochloropsis Oculata For Use In Tropical Aquaculture. Journal of Applied Phycology. 3: 43-53.
A. Converti, A. A. Casazza, E. Y. Ortiz, P. PeregoB. M. Del. 2009. Effect Of Temperature And Nitrogen Concentration On The Growth And Lipid Content Of Nannochloropsis Oculata And Chlorella Vulgaris For Biodiesel Production. Chemical Engineering and Processing: Process Intensification. 48: 1146-1151.
S. Y. Chiu, C. Y. Kao, M. T. Tsai, S. C. Ong, C. H. ChenC. S. Lin. 2009. Lipid Accumulation And CO2 Utilization Of Nannochloropsis Oculata In Response To CO2 Aeration. Bioresource Technology. 100: 833-838.
B. Gianluca, B. Marco, C. Benedetta de, F. Paolo deS. Marco. 2013. Effect Of Nitrogen And Phosphorus Starvations On Chlorella Vulgaris Lipids Productivity And Quality Under Different Trophic Regimens For Biodiesel Production. American Journal of Plant Sciences. 4: 44-51.
M. Chen, H. Tang, H. Ma, T. C. Holland, K. Y. S. NgS. O. Salley. 2011. Effect Of Nutrients On Growth And Lipid Accumulation In The Green Algae Dunaliella Tertiolecta. Bioresource Technology. 102: 1649-1655.
M. Takagi, K. Watanabe, K. YamaberiT. Yoshida. 2000. Limited Feeding Of Potassium Nitrate For Intracellular Lipid And Triglyceride Accumulation Of Nannochloris Sp. UTEX LB1999. Applied Microbiology and Biotechnology. 54: 112-117.
S. Ruangsomboon. 2012. Effect Of Light, Nutrient, Cultivation Time And Salinity On Lipid Production Of Newly Isolated Strain Of The Green Microalga, Botryococcus Braunii KMITL 2. Bioresource Technology. 109: 261-265.
M. Azma, M. S. Mohamed, R. Mohamad, R. A. RahimA. B. Ariff. 2011. Improvement Of Medium Composition For Heterotrophic Cultivation Of Green Microalgae, Tetraselmis Suecica, Using Response Surface Methodology. Biochemical Engineering Journal. 53: 187-195.
J. Y. Lee, C. Yoo, S. Y. Jun, C. Y. AhnH. M. Oh. 2010. Comparison Of Several Methods For Effective Lipid Extraction From Microalgae. Bioresource Technology. 101: S75-S77.
E. G. BlighW. J. Dyer. 1959. Extraction of Lipids in Solution by the Method of Bligh & Dyer. Biochemistry Physiology 911-917.
H. Sharif., S. Aishah., N. B. Amru, C. ParthaN. Mohd. 2008. Biodiesel Fuel Production From Algae As Renewable Energy. American Journal of Biochemistry and Biotechnology. 4: 250-254.
F. Steffii, M. BrendanN. Erin. 2003. Nitrate And Phosphate Levels Positively Affect The Growth Of Algae Species Found In Perry Pond. Grinnell College. 4: 21-24.
E. W. Becker. 1994. Microalgae: Biotechnology and MicrobiologyCambridge. University Press. 293.
F. Pingzhong, D. Zhongyang, F. LuH. Zhengyu. 2012. Lipid Accumulation And Growth Characteristics Of Chlorella Zofingiensisunder Different Nitrate And Phosphate Concentrations. Journal of Biosciences and Bioengineering. 114: 405-410.
K. S. Kalpesh, S. HolgerM. S. Peer. 2012. High Lipid Induction In Microalgae For Biodiesel Production. Journal Energies. 5: 1532-1553.
S. Rasoul-Amini, N. Montazeri-Najafabady, M. A. Mobasher, S. Hoseini-AlhashemiY. Ghasemi. 2011. Chlorella sp.: A New Strain With Highly Saturated Fatty Acids For Biodiesel Production In Bubble-Column Photobioreactor. Applied Energy. 88: 3354-3356.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.