COMPARISON OF ALLERGENIC PROTEINS OF SEA SNAIL (CERITHIDEA OBTUSA) AND FRESHWATER SNAIL (POMACEA CANALICULATA)
DOI:
https://doi.org/10.11113/.v78.7940Keywords:
Cerithidea obtusa, Pomacea canaliculata, snail, allergen, SDS-PAGE, immunoblottingAbstract
In Malaysian and certain Asian countries, snail has high market demand and popular to the local people as food. However, snail is also frequently reported as one of the worst food allergens, dominated by severe symptoms such as asthma and anaphylactic shock. Thus, the aims of this study is to determine the allergenicity of two species of edible snails; the local sea snail, Cerithidea obtusa and the freshwater snail Pomacea canaliculata. Snail extracts were prepared from the snail flesh and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine their protein profiling. Allergenic proteins were detected by immunoblotting test using sera from 10 snail-allergic patients. The snails contain 31 to 34 protein fractions between 11 to >250 kDa. The prominent bands were seen at 33, 42, 74 and 250 kDa. Immunoblotting detected 15 and 16 allergenic proteins in C. obtusa and P. canaliculata, respectively. Three protein fractions at 30, 33 and 42 kDa were identified as the major allergens of C. obtusa, while six major allergens at 30, 33, 42, 74, 124 and 218 kDa were detected in P. canaliculata. Various minor allergens were also detected in both snails. This study indicated that numerous proteins of C. obtusa and P. canaliculata were allergenic. Thus, combined allergen extracts of both snails are essential to be included in diagnosis of snail allergy among local allergic patients. Â
References
Taylor, S. L. 2008. Molluscan Shellfish Allergy. Advances in Food and Nutrition Research. 54: 140-177. doi: 10.1016/S1043-4526(07)00004-6.
de la Cuesta, C. G., GarcÃa, B. E., Córdoba, H., Diéguez, I. and Oehling, A. 1989. Food Allergy to Helix terrestre (Snail). Allergollogia et Immunopathologia. 17(6): 337-9.
Purwaningsih, S., Suseno, S. H., Salamah, E., Mulyaningtyas, J. R. and Dewi, Y. P. 2015. Effect Of Boiling And Steaming On The Profile Fatty Acids And Cholesterol In Muscle Tissue Of Molluscs. International Food Research Journal. 22(3): 1087-1094.
Popescu, F-D. 2015. Cross-Reactivity Between Aeroallergens And Food Allergens. World Journal of Methodology. 5(2): 31-50. doi: 10.5662/wjm.v5.i2.31.
Bhattacharya, S., Chakraborty, M., Bose, M., Mukherjee, D., Roychoudhury, A., Dhar, P., and Mishra, R. 2014. Indian Freshwater Edible Snail Bellamya Bengalensis Lipid Extract Prevents T Cell Mediated Hypersensitivity And Inhibits LPS Induced Macrophage Activation. Journal of Ethnopharmacology. 157: 320-9. doi: 10.1016/j.jep.2014.09.009.
Martins, L., Pires, E. and Ináicio, F. 2009. CD4 T-Cell Cytokine Response To Mite Recombinant Tropomyosin In Mite, Snail And Shrimp Allergic Patients. Journal of Allergy, Asthma and Immunology. 7(1): 1-22.
Pajno, G. B., La Grutta, S., Barberio, G., Canonica, G. W., and Passalacqua, G. 2002. Harmful Effect Of Immunotherapy In Children With Combined Snail And Mite Allergy. Journal of Allergy, Asthma and Immunology. 109(4): 627-629.
Calderón, M. A., Linneberg, A., Kleine-Tebbe, J., De Blay, F., Hernandez Fernandez de Rojas, D., Virchow, J. C. and Demoly, P. 2015. Respiratory Allergy Caused By House Dust Mites: What Do We Really Know? Journal of Allergy and Clinical Immunology. 136(1): 38-48. doi: 10.1016/j.jaci.2014.10.012.
van Ree, R., Antonicelli, L., Akkerdaas, J. H., Pajno, G. B., Barberio, G., Corbetta, L., Ferro, G., Zambito, M., Garritani, M. S., Aalberse, R. C. and Bonifazi, F. 1996. Asthma After Consumption Of Snails In House-Dust-Mite-Allergic Patients: A Case Of Ige Cross-Reactivity. Allergy. 51(6): 387-393.
Woo, C. K., and Bahna, S. L. 2011. Not All Shellfish “Allergy†Is Allergy! Clinical and Translational Allergy. 1: 3.
Ishikawa, M., Ishida, M., Shimakura, K., Nagashima, Y., and Shiomi, K. 1998. Purification And Ige-Binding Epitopes Of A Major Allergen In The Gastropod Turbo cornutus. Bioscience, Biotechnology and Biochemistry. 62(7): 1337-1343.
Asturias, J. A., Eraso, E., Arilla, M. C., Gomez-Bayon, N., Inacio, F., and Martinez, A. 2002. Cloning, Isolation And Ige-Binding Properties Of Helix Aspersa (Brown Garden Snail) Tropomyosin. International Achieves of Allergy and Immunology. 128: 90-96.
Bessot, J. C., Metz-Favre, C., Rame, J. M., Blay, F. D., and Pauli, G. 2010. Tropomyosin Or Not Tropomyosin, What Is The Relevant Allergen In House Dust Mite And Snail Cross Allergies? European Annals of Allergy and Clinical Immunology. 42(1): 3-10.
Lopata, A. L., and Lehrer, S. B. 2009. New Insights Into Seafood Allergy. Current Opinion of Allergy and Clinical Immunology. 9: 270-277. doi: 10.1097/ACI.0b013e32832b3e6f.
WoRMS Editorial Board. 2016. World Register of Marine Species. Available from http://www.marinespecies.org at VLIZ. Accessed 2016-03-15.
Mohd Salleh, N. H., Arbain, D., Mohamed Daud, M. Z., Pilus, N., Naw, R. 2012. Distribution And Management Of Pomacea Canaliculata In The Northern Region Of Malaysia: Mini Review. APCBEE Procedia. 2: 129-134.
Zailatul, H. M. Y., Rosmilah, M., Faizal, B., Noormalin, A., and Shahnaz, M. 2015. Malaysian Cockle (Anadara Granosa) Allergy: Identification Of Ige-Binding Proteins And Effects Of Different Cooking Methods. Tropical Biomedicine. 32(2): 323-334.
Yadzir, Z. H., Misnan, R., Bakhtiar, F., Abdullah, N., and Murad, S. 2015. Tropomyosin, The Major Tropical Oyster Crassostrea Belcheri Allergen And Effect Of Cooking On Its Allergenicity. Allergy, Asthma and Clinical Immunology. 11: 30. doi: 10.1186/s13223-015-0099-4.
Hauser, M., Roulias, A., Ferreira, F., and Egger, M. 2010. Panallergens And Their Impact On The Allergic Patient. Allergy, Asthma & Clinical Immunology. 6: 1. doi: 10.1186/1710-1492-6-1.
Granum, B., and Løvik, M. 2002. The Effect Of Particles On Allergic Immune Responses. Toxicological Sciences. 65(1): 7-17.
Leung, N. Y., Wai, C. Y., Shu, S., Wang, J., Kenny, T. P., Chu, K. H., and Leung, P. S. 2014. Current Immunological And Molecular Biological Perspectives On Seafood Allergy: A Comprehensive Review. Clinical Reviews in Allergy and Immunology. 46(3): 180-197. doi: 10.1007/s12016-012-8336-9.
Rosmilah, M., Shahnaz, M., Zailatul Hani, M. Y., and Noormalin, A. 2012. Identification Of Tropomyosin And Arginine Kinase As Major Allergens Of Portunus pelagicus (Blue Swimming Crab). Tropical Biomedicine. 29(3): 467-478.
Mohamad Yadzir, Z. H., Misnan, R., Bakhtiar, F., Abdullah, N., and Murad, S. 2015. Tropomyosin And Actin Identified As Major Allergens Of The Carpet Clam (Paphia Textile) And The Effect Of Cooking On Their Allergenicity. Biomedical Research International. 2015: 2 54152. doi: 10.1155/2015/254152.
Bauermeister, K., Wangorsch, A., Garoffo, L. P., Reuter, A., Conti, A., Taylor, S. L., Lidholm, J., Dewitt, A. M., Enrique, E., Vieths, S., Holzhauser, T., Ballmer-Weber, B., and Reese, G. 2011. Generation Of A Comprehensive Panel Of Crustacean Allergens From The North Sea Shrimp Crangon Crangon. Molecular Immunology. 48(15-16): 1983-92. doi: 10.1016/j.molimm.2011.06.216.
Suzuki, M., Kobayashi, Y., Hirakia, Y., Nakata, H., and Shiomi, K. 2011. Paramyosin Of The Disc Abalone Haliotis Discus Discus: Identification As A New Allergen And Cross-Reactivity With Tropomyosin. Food Chemistry. 124(3): 921-926.
Lockey, R. F., and Ledford, D. K. 2014. Allergens And Allergen Immunotherapy: Subcutaneous, Sublingual And Oral. Fifth Edition. New York: CRC Press.
Samson, K. T., Chen, F. H., Miura, K., Odajima, Y., Iikura, Y., Naval Rivas, M., Minoguchi, K., and Adachi, M. 2004. IgE Binding To Raw And Boiled Shrimp Proteins In Atopic And Nonatopic Patients With Adverse Reactions To Shrimp. International Archives of Allergy and Immunology. 133(3): 225-32.
Barber, D., Polo, F., Lombardero, M., Villalba, M., and RodrÃguez, R. 2006. The Importance Of Minor Allergens In Allergen Standardization. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A. M. 95: 128-134.
Lasne, F. 2009. Double-Blotting: A Solution To The Problem Of Nonspecific Binding Of Secondary Antibodies In Immunoblotting Procedures. Methods in Molecular Biology. 536: 213-219. 95:128-134. doi: 10.1007/978-1-59745-542-8_23.
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