PHYLOGENETIC ANALYSIS OF LOCAL ENDEMIC BUFFALO (BUBALUS BUBALIS) BASED ON CYTOCHROME B GENE IN CENTRAL INDONESIA
DOI:
https://doi.org/10.11113/jt.v78.8343Keywords:
Bubalus bubalis, cytochrome B gene, phylogenetic treeAbstract
Buffalo population in the central of Indonesia such as Bali, NTB, and Sulawesi is decreased. Cause of the decline of buffalo population is inbreeding. For conservation efforts to avoid inbreeding required the identification of buffalo genetic variations on molecular scale as phylogeny analysis with cyt b gene. The purpose of this study were to reconstruct the phylogenetic tree of buffalo ((Bubalus bubalis) in the central of Indonesia with cyt b gene. Animal materials were collected from four different area: Bali, Lombok, Bima and Toraja. DNA isolation, PCR (polymerase chain reaction) was conducted under standar protocols. Sequence from four representaive animals based on area were used to contruct the phylogenetic tree and haplotype network. Analyses of plylogenetic tree showed that the reconstruction has one major clade and two sub clade. It showed that buffalo in central of Indonesia had close genetic relationship.
References
Aminafshar, M. 2008. Genetic Deversity in Buffalo Population of Guilan Using Microsatellite Markers. Journal Of animal and veterinary Advances. Vol 7 (11): 1499-1502. (Online),(http://www.researchgate.net/profile/Cyrus_Amirinia/publication/26590381_Genetic_Diversity_in_Buffalo_Population_of_Guilan_Using_Microsatellite_Markers/links/02e7e537049075d06c000000.pdf,) diakses pada tanggal 19 september 2014.
Arnason, U, dan Gullberg A. 1996. Cytochrome b nucleotide sequences and the identification f five primary lineage of Extant cetaceans. Mol Biol Evol, 13(2):407-417. (Online), (http://mbe.library.arizona. edu/ data/1996/1302/11arna.pdf), diakses pada tanggal 19 September 2015.
Ditjennak.2011.Populasi nasional ternak kerbau tahun 2002 s/d2011. (Online), (http://ditjennak.pertanian.go.id/), diakses pada tanggal 2 September 2014.
Ellegren. 2004. Microsatellites Simple Sequences With Complex Evolution Genetics. (Online), (http://www. as. wvu. edu/~s difazio/ molececol/ Sep13b. pdf.), diakes pada tanggal 20 September 2014.
Erdiansyah, E, dan Anneke A. 2008. Keragaman Fenotipe Dan Pendugaan Jarak Genetik Antara Subpopulasi Kerbau Rawa Lokal Di Kabupaten Dompu, Nusa Tenggara Barat. Seminar dan Lokakarya Nasional Usaha Ternak Kerbau 2008. (Online), (http:// peternakan. itbang. pertanian. go. Id / fullteks / lokakarya / lkbo08-6.pdf?secure=1), diakses pada tanggal 30 Maret 2015.
Hsieh, H, Tsai, K, Meng, Linacre, L. 2003. Species Identification Of Rhinoceros Horns Using the Cytochrome b. Forensic science International 2003 Sep 9;136(1-3):1-11.
Kornegay, J. R.; Kocher, T. D.; Williams, L. A. and Wilson, A. C. 1993. Pathways of lysozyme evolution inferred from the sequences of cytochrome-b in birds. J. Mol. Evol. 37: 367-379
Krajewski, C. and King, D. G. 1996. Molecular Divergence and Phylogeny: Rates and Patterns of Cytochrome-b Evolution in Cranes. Mol. Biol. Evol. 13(1): 21-30.
Mirhabibie, Manafiazar, Qaravisi, dan Mahmoodi. 2007. Inbreeding and its effect on soe productive traits in buffaloes of south Iran. Journal Animal Science, Vol 06 (suppl, 2):372-373. (Online), (http:// www. Aspajournal . it / index. Php / ijas / article / viewFile/ijas.2007.s2.372/967), diakses pada tanggal 20 September 2014.
Moore, W. S. and DeFilippis, V. R. 1997. The window of taxonomic resolution for phylogenies based on mitochondrial cytochrome-b. In: Avian Molecular Evolution and Systematic. Ed. by David P. Mindell. Academic Press: California. p. 84-107
Motokawa, M.; Suzuki, H.; Harada, M.; Lin, L-K.; Koyasu, K. and Oda, S-I. 2000. Phylogenetic relationships among East Asian species of Crocidura (Mammalia, Insectivora) inferred from mitochondrial cytochrome-b gene sequences. Zoo. Sci. 17: 497-504
Okazaki, T.; Jeon, S. R.; Watanabe, M. and Kitagawa, T. 1999. Genetic relationships of Japanese and Korea Bagrid Catfishes inferred from mitochondrial DNA analysis. Zoo. Sci. 16: 363-373.
Oshida, T.; Lin, L-K.; Masuda, R. and Yoshida, M. C. 2000b. Phylogenetic relationships among Asian species of Petaurista (Rodentia, Sciuridae), inferred from mitochondrial cytochrome-b gene sequences. Zoo. Sci. 17: 123-128
Pakedorf, and Stoneking. 2005. Mitochondrial DNA and human evolution, Annu. Rev. Genom. Human. Genet Vol 6: 165-183.
Saenab. 2009. Pemetaan variasi genetk kerbau lokal tana toraja berbasis restriction Fragment Length polymorphsm-Dna (RFLP-DNA) sebagai sumer belajar di sekolah menengah atas dan perguruaan tinggi. Tesis tidak Diterbitkan. Malang :Program Pascasarjana Universitas Negeri Malang
Sahilah, A, Norhayati Y, Norrakiah A, Aminah A. and Wan Aida. 2011. Halal authentication of raw meats using PCR amplification of mitchondrial DNA. International Food Research Journal 18(4): 1489-1491 (2011). (online), (http: // www . ifrj. upm. edu. my / 18 % 20%2804%29%202011/%2839%29IFRJ-2011-025.pdf, diakses pada tanggal 30 Maret 2015).
Sukri, A. 2011. Identifikasi variassi genetic kerbau lokal (Bubalus bubalis) Lombok Tengah , Nusa Tenggara Barat Berbasis Mikrosatelit Sebagai bahan Ajar Matakuliah Genetika. Tesis tidak diterbitkan. Pascasarjana universita Negeri Malang.
Sukri, A. 2014. Analisis Fiologeni Kerbau Lokal Indoonesia (Bubalus bubalis) dengan Gen cyt b Berbasis Biogeografi sebagai bahan ajar Matakuliah Bioinformatika. Disertasi universitas Negeri Malang Pascasarjana Program Studi Pendidikan Biologi juni 2014.
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
