CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE
DOI:
https://doi.org/10.11113/jt.v78.9945Keywords:
Aliphatic hydrocarbon, consortium, PAHs, n-alkane degraderAbstract
Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.References
Ojo, O. A. 2006. Petroleum-Hydrocarbon Utilization by Native Bacterial Population from a Wastewater Canal Southwest Nigeria. African Journal of Biotechnology. 5(4): 333-337.
Wise, D. L., and Trantolo, D. J. 1994. Remediation of Hazardous Waste Contaminated Soils. Series 8. New York: Marcel Dekker Inc.
Ilyina, A., Castillo, S. M. I., Villarreal, S. J. A., Ramirez, E. G., and Candelas, R. J. 2003. Isolation of Soil Bacteria for Bioremediation of Hydrocarbon Contamination. Becth Mock Yh-Ta. (44): 88-91.
Calabrese, E., J., and P. T. Kostecki. 1991. Petroleum Contaminated Soils. 2. Chelsea MI: Lewis publishers, Inc.
Mrozik, A., Seget, Z. P., and Labuzek, S. 2003. Bacterial Degradation and Bioremediation of Polycyclic Aromatic Hydrocarbons. Polish Journal of Environmental Studies. 12: 15-25.
Roling, W. F. M., Milner, M. G., Jones, D. M., Lee, K., Daniel, F., Swannell, R. J. and Head, I. M. 2002. Robust Hydrocarbon Degradation And Dynamics Of Bacterial Communities During Nutrient-Enhanced Oil Spill Bioremediation. Applied and Environmental Microbiology. 68(11): 5537-5548.
Ramsay, M. A., Swannell, R. P. J., Shipton, W. A., Duke, N. C. and Hill, R. T. 2000. Effect of Bioremediation on the Microbial Community in Oiled Mangrove Sediments. Marine Pollution Bulletin. 41: 413-419.
Engebrecht, J., R. Brent, and Kaderbhai, M. A. 1992. Minipreps of Plasmid DNA. In Ausubel, F., M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (ed). Short Protocols in Molecular Biology. New York: Greene Publishing Associates & Wiley.
Edwards, U., Rogall, T., Blocker, H., Emde, M., and Bottger, E. C. 1989. Isolation and Direct Complete Nucleotide Determination of Entire Genes. Characterization of a Gene Coding for 16s ribosomal RNA. Nucleic Acid Research. 17(9): 7843 - 7853.
Boone, R. D., D. F. Grigal, P. Sollins, R. J. Ahrens, and D. E Armstrong. 1996. Soil sampling, preparation, archiving and quality control. In Tan, K. H (ed.) Soil Sampling, Preparation, and Analysis. Marcel Dekker, Inc. New York.
Grace, C., H. Murray, and C. B. Phil. 2006. Laboratory Manual of the Soil Microbial Biomoass Group. Methods: Rothamsted Research.
Brown, R. B. 2003. Soil Texture. Soil Science Fact Sheet SL-29. Florida: University of Florida Institute of Food and Agricultural Sciences (IFAS) Extension Service, Gainesville. SL29.
Adoki, A., and Orugbani, T. 2007. Removal of Crude Petroleum Hydrocarbons by Heterotrophic Bacteria in Soils Amended with Nitrogenous Fertilizer Plant Effluents. African Journal of Biotechnology. 6 (13): 1529-1535.
Howsam, H. W. and Puttmann, W. 1989. Petrographic and Geochemical Properties of Selected South Brazilian Coals. Zentralblatt fur Geologie und Palaontologie. Part 1 (9/10): 1565-1573.
Nweke, C. O., and Okpokwasili, G. C. 2003. Drilling Fluid Base oil Biodegradation Potential of a Soil Staphylococcus Species. African Journal of Biotechnology. 2(9): 293-295.
Odokuma, L. O., and Dickson, A. A. 2003. Bioremediation of a Crude Oil Polluted Tropical Rain Forest Soil. Global Journal of Environmental Sciences. 2: 29-40.
Nkwelang, G., Kamga, H. F. L., Nkeng, G. E., and Antai, S. P. 2008. Studies on the Diversity, Abundance and Succession of Hydrocarbon Utilizing Microorganisms in Tropical Soil Polluted with Oily Sludge. African Journal of Biotechnology. 7(8): 1075-1080
Downloads
Published
Issue
Section
License
Copyright of articles that appear in Jurnal Teknologi belongs exclusively to Penerbit Universiti Teknologi Malaysia (Penerbit UTM Press). This copyright covers the rights to reproduce the article, including reprints, electronic reproductions, or any other reproductions of similar nature.
